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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,004 results for: URE

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Showing: 1281 - 1300 of 18004 results

  7. MSP2440

    Plasmid
    #72250
    Purpose
    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72250-plasmid-map-sequence-id-131871

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  14. K-to-R mutant NPRL2(1-380) in pLJM60

    Plasmid
    #184562
    Purpose
    CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.
    Depositor
    Kacper Rogala
    Article
    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:
    Insert
    NPRL2 (NPRL2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    All lysines were mutated to arginines.
    Promoter
    CMV
    Available Since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184562-plasmid-map-sequence-id-376960

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  15. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid
    #101217
    Purpose
    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101217-plasmid-map-sequence-id-197215

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Showing: 1281 - 1300 of 18004 results