We narrowed to 13,620 results for: crispr cas9

1. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

   Plasmid

   #124859

   Purpose

   Mutagenesis of Slc17a7

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a7 (Slc17a7 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

   Plasmid

   #169912

   Purpose

   Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   dCjCas9
   

   Use

   CRISPR

   Tags

   10XGCN4, 2xSV40NLS, and SV40NLS-HA

   Expression

   Mammalian

   Mutation

   D8A, H559A in cjCas9

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.3-Nm-dCas9-p300 Core

   Plasmid

   #61365

   Purpose

   encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   Nm-dCas9-p300 Core (EP300 Synthetic, Neisseria meningiditis, Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D16A, D587A, H588A, N611A in Nm Cas9

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-p300 PHD-HAT

   Plasmid

   #179545

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWalium20-10XUAS-3XFLAG-dCas9-VPR

   Plasmid

   #78897

   Purpose

   Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

   Depositor

   Norbert Perrimon

   Article

   Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

   Insert

   dCas9-VPR
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   10XUAS

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. D10A-Cas9

   Plasmid

   #239383

   Purpose

   For circular RNA-mediated inverse prime editor using nCas9-D10A in HEK294T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

   Insert

   nCas9-D10A
   

   Use

   CRISPR

   Tags

   BPNLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A in Cas9

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-dCas9-dTET1-H1672Y-D1674A-Hygro

   Plasmid

   #104413

   Purpose

   transient expression of dCas9-dTET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   dTET1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   H1672Y-D1674A

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX400 Campylobacter lari CF89-12 Cas9

   Plasmid

   #68336

   Purpose

   Campylobacter lari CF89-12 Cas9

   Depositor

   Feng Zhang

   Insert

   ClCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-dCas9-JMJD2A-S288G-T289G-Hygro

   Plasmid

   #104418

   Purpose

   transient expression of dCas9-dJMJD2A fusion protein

   Depositor

   Stephan Beck

   Insert

   dJMJD2A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   S288G-T289G

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX399 Mycoplasma gallisepticum str. F Cas9

    Plasmid

    #68335

    Purpose

    Mycoplasma gallisepticum str. F Cas9

    Depositor

    Feng Zhang

    Insert

    MgCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149426

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[Sxl, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149427

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[TraB, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid

    #74408

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. hPLD3-sgRNA-Cas9_mcherry

    Plasmid

    #199342

    Purpose

    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. hPLD4-sgRNA-Cas9_mcherry

    Plasmid

    #199343

    Purpose

    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    sgRNA: accagtagtatgaagccacg

    Promoter

    human U6

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PiggyBac dCas9-KRAB

    Plasmid

    #191314

    Purpose

    piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    KRAB-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScI_dCas9-CDA_J23119-sgRNA

    Plasmid

    #108550

    Purpose

    Bacterial Target-AID vector with sgRNA

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Inserts

    dCas9-PmCDA1
    Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    J23119 and lambda OR

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE TRE dCas9

    Plasmid

    #50915

    Purpose

    Tet-regulatable dCas9 lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-cas9-2A-mCherry

    Plasmid

    #107592

    Purpose

    An entry vector with cas9 -2A-mCherry

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    cas9-2A-mCherry
    

    Use

    CRISPR

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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