Addgene

We narrowed to 6,478 results for: sas

Showing: 1281 - 1300 of 6478 results

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pcDNA3.1-ERBB4 (E563K)

Plasmid

#29532

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed glutamic acid 563 to lysine

Promoter

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107322

Purpose

Expresses SpCas9 (R1335K) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to SaCas9

Promoter

CMV IE93

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9MT3-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107325

Purpose

Expresses SpCas9 (R1335K) fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to NmCas9

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9MT2-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107326

Purpose

Expresses SpCas9 (R1333S) fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to NmCas9

Promoter

CMV IE93

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (E563K)

Plasmid

#29541

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

Plasmid

#69232

Purpose

Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-LRTM2

Plasmid

#69237

Purpose

U6 driven SpCas9 sgRNA expression for LRTM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

LRTM2 sgRNA (LRTM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD4_1

Plasmid

#36374

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD4 (MBD4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (R544W)

Plasmid

#29540

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
p300dAIL KAT LIC 1M

Plasmid

#233588

Purpose

Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

Depositor

Scott Showalter

Article

Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

Insert

p300dAIL KAT (EP300 Human)

Use

Tags

6xHis MBP

Expression

Bacterial

Mutation

deleted amino acids 1523-1554

Promoter

T7

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pVeBtA3

Plasmid

#238988

Purpose

Mammalian expression of bovine arrestin-3 short isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

Insert

arrestin-3 short (ARR3 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pVeBtA1

Plasmid

#238986

Purpose

Mammalian expression of bovine arrestin-1 with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

Insert

arrestin-1 (SAG Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pVeBtA2

Plasmid

#238987

Purpose

Mammalian expression of bovine arrestin-2 long isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

Insert

arrestin-2 long (ARRB2 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pVeBtA2-3A

Plasmid

#238991

Purpose

Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

Insert

arrestin-2 long (ARRB2 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

I386A,V387A,F388A

Promoter

CMV

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBtA3

Plasmid

#238985

Purpose

Mammalian expression of bovine arrestin-3 short isoform

Depositor

Vsevolod Gurevich

Article

Hanson et al J Mol Biol. 2007 Apr 27;368(2):375-87. d

Insert

arrestin-3 short (ARR3 Bovine)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2 Cys-less

Plasmid

#236273

Purpose

Bacterial expression of bovine arrestin-2 C59V, C125S, C140L, C150V, C242V, C251V, C269S

Depositor

Vsevolod Gurevich

Article

Zhuo et al J Biol Chem. 2014 Jul 25;289(30):20991-1

Insert

arrestin-2 (ARRB2 Bovine)

Use

Tags

Expression

Bacterial

Mutation

C59V, C125S, C140L, C150V, C242V, C251V, C269S

Promoter

lac

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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p3HM1Luc4

Plasmid

#236277

Purpose

Mammalian expression of human muscarinic M1 receptor; N-terminal HA tag, C-terminal Rluc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

muscarinic M1 receptor (CHRM1 Human)

Use

Tags

3xHA and Rluc8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Perturb-FISH

Plasmid

#220626

Purpose

expression of gRNAs under U6T7 promoter

Depositor

Samouil Farhi

Article

Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

BsmBI

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCfB8589

Plasmid

#175221

Purpose

Xenopus oocyte expression vector containing YMR253C. Contains T7 RNA polymerase binding site, and adds polyA tail to YMR253C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

Depositor

Irina Borodina

Article

Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

Insert

YMR253C

Use

Mrna expression, injection into xenopus oocytes

Tags

PolyA

Expression

Mutation

Promoter

T7

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8707

Plasmid

#175222

Purpose

Xenopus oocyte expression vector containing YNL095C. Contains T7 RNA polymerase binding site, and adds polyA tail to YNL095C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

Depositor

Irina Borodina

Article

Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

Insert

YNL095C

Use

Mrna expression, injection into xenopus oocytes

Tags

PolyA

Expression

Mutation

Promoter

T7

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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