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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 1301 - 1320 of 3270 results

  2. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Publication
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113035-plasmid-map-sequence-id-224728

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  3. pMXS-GshF-blasticidin

    Plasmid
    #210313
    Purpose
    Expresses humanized Streptococcus thermophilus GshF in mammalian cells
    Depositor
    Kivanc Birsoy
    Publication
    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:
    Insert
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    Use
    Retroviral
    Tags
    FLAG peptide
    Mutation
    Codon optimized for expression in human cells
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    210313-plasmid-map-sequence-id-418847

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  5. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #129418
    Purpose
    Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 and sgRAB18.N (RAB18 Human)
    Use
    CRISPR
    Tags
    T2A-mCherry-P2A-Puro
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129418-plasmid-map-sequence-id-254820

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  7. pRS416gT-GalL-Cas9

    Plasmid
    #79903
    Purpose
    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression
    Depositor
    Ronald Davis
    Publication
    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw
    Inserts
    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GPM1 promoter, GalL, and RPR1 promoter with TetO …
    Available since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    79903-plasmid-map-sequence-id-157872

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  10. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Publication
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Tags
    3x-FLAG
    Expression
    Bacterial
    Promoter
    pTet
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

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  11. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid
    #155050
    Purpose
    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    Use
    CRISPR
    Available since
    Oct. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155050-plasmid-map-sequence-id-315172

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  12. Topo_SpCas9.tracr_LbCas12a.DR

    Plasmid
    #155049
    Purpose
    TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
    Use
    CRISPR
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155049-plasmid-map-sequence-id-308669

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  15. pUC19-F3UXG6

    Plasmid
    #103108
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103108-plasmid-map-sequence-id-201817

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  18. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Markus Schmid
    Publication
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Mutation
    Contains D10A and H840A mutations
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122836-plasmid-map-sequence-id-239365

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  19. CBE6b-NRCH

    Plasmid
    #257827
    Purpose
    CBE6b Sp Cas9 NRCH IVT plasmid
    Depositor
    Gregory Newby
    Insert
    CBE6b SpCas9 NRCH
    Expression
    Mammalian
    Available since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    257827-plasmid-map-sequence-id-513601

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Showing: 1301 - 1320 of 3270 results