We narrowed to 3,240 results for: Streptococcus
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Plasmid#155050PurposeTOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vectorDepositorInsertSpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
UseCRISPRAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Topo_SpCas9.tracr_LbCas12a.DR
Plasmid#155049PurposeTOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vectorDepositorInsertSpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
UseCRISPRAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCF730
Plasmid#121668PurposeEF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW-AlphaLobe
Plasmid#113255PurposeE. coli expression vector for His-MBP-Cas9 Alpha-Helical LobeDepositorInsertS. pyogenes Cas9 alpha-helical lobe
Tags10xHis-MBPExpressionBacterialMutationresidues 56–714; See paper for descriptionPromoterT7Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-F3UXG6
Plasmid#103108PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertF3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
phumanCas9 (GB0575)
Plasmid#68206PurposeProvides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid partDepositorInsertCas9 coding region (human codon optimised)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removed; human codon optimis…Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SpG-His
Plasmid#179318PurposePlasmid for bacterial expression and purification of SpG-Cas9DepositorInsertHuman codon-optimized Cas9 variant SpG
UseCRISPRTags6xHis and SV40-NLSExpressionBacterialMutationSpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337RAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH220
Plasmid#122836PurposedCas9 with GreenGate B-C flanking sequencesDepositorInsertNuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorMutationContains D10A and H840A mutationsAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CBE6b-NRCH
Plasmid#257827PurposeCBE6b Sp Cas9 NRCH IVT plasmidDepositorInsertCBE6b SpCas9 NRCH
ExpressionMammalianAvailable sinceJune 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX458-MAFB-sg
Plasmid#194720Purposepx458 with guide RNA that target hMAFBDepositorAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJCC_162 SpCas9 KES(1107-1109)GG
Plasmid#179526PurposeFor bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 KES(1107-1109)GG
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationreplaced KES (residues 1107-1109) with GGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_163 SpCas9 K233A/K234A/K253A/K263A
Plasmid#179527PurposeFor bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 K233A/K234A/K253A/K263A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged lysines 233, 234, 253, and 263 to alanineAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
UC16m
Plasmid#121038PurposeMoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.DepositorInsertgQi Cas9 gRNA UC part
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
UC17m
Plasmid#121039PurposeMoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertgV1 Cas9 gRNA UC part
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV521
Plasmid#132346PurposeCas9 R promoter targeting sgRNA1 expression cassette for Zea maysDepositorInsertCas9 R promoter targeting sgRNA1
ExpressionPlantAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PV523
Plasmid#132347PurposeCas9 R promoter targeting sgRNA2 expression cassette for Zea maysDepositorInsertCas9 R promoter targeting sgRNA2
ExpressionPlantAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PV528
Plasmid#132348PurposeCas9 R promoter targeting sgRNA3 expression cassette for Zea maysDepositorInsertCas9 R promoter targeting sgRNA3
ExpressionPlantAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPhygSaCas9
Plasmid#123262PurposeExpresses Staphylococcus aureus Cas9 (SaCas9) in LeishmaniaDepositorInsertStaphylococcus aureus Cas9 (NEWENTRY )
UseCRISPR; LeishmaniaAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 5
Plasmid#51764PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 5
UseCRISPR and LentiviralPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA
Plasmid#133334Purposefor constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRPromoterconstitutiveAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only