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173,176 results

Showing: 2641 - 2680 of 173176 results

  1. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid
    #162279
    Purpose
    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN2A Variant 1, stabilized (SCN2A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp4551.
    Promoter
    CMV
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162279-plasmid-map-sequence-id-321873

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  2. eSpCas9(1.1)-T2A-Puro

    Plasmid
    #101039
    Purpose
    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
    Depositor
    Andrea Németh
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3xFLAG and T2A-Puro
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    101039-plasmid-map-sequence-id-190363

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  9. S17-1λpir gyrAR462C

    Bacterial Strain
    #237425
    Purpose
    This engineered E. coli S17-1 λpir strain, featuring a mutation in the gyrA gene (462Arg→Cys), was designed to confer resistance to the CcdB toxin, allowing it to survive with a ccdB-carrying plasmid.
    Depositor
    Xue Liu
    Article
    Zhang et al Nat Commun. 2025 Jul 1;16(1):5870. doi:
    Bacterial Resistance
    None
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only

  10. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  11. pD649-HAsp-LILRB2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156863
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    LILRB2 (LILRB2 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    156863-plasmid-map-sequence-id-382983

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  12. pRVL-6

    Plasmid
    #104584
    Purpose
    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and K322A/P331S
    Promoter
    CMV
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104584-plasmid-map-sequence-id-198608

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  14. pmCherry Paxillin

    Plasmid
    #50526
    Depositor
    Kenneth Yamada
    Insert
    PXN paxillin (PXN Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50526-plasmid-map-sequence-id-241928

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  15. pAAV-hSyn1-eYFP (AAV9)

    Viral Prep
    #117382-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    Syn
    Tags
    eYFP
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  16. pCS2-UTX-F-MT2

    Plasmid
    #40619
    Purpose
    Expression of enzyme-dead UTX in mammalian cells
    Depositor
    Kai Ge
    Article
    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.
    Insert
    UTX (KDM6A Human)
    Tags
    6xMyc and Flag
    Expression
    Mammalian
    Mutation
    changed Histidine 1146 and Glutamic Acid 1148 to …
    Promoter
    CMV
    Available Since
    Nov. 5, 2012
    Availability
    Academic Institutions and Nonprofits only

  25. Ydf NanoLuc

    Plasmid
    #225656
    Purpose
    Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NanoLuciferase
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Promoter
    PydfJ115
    Available Since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225656-plasmid-map-sequence-id-455061

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  36. pVITRO1-Trastuzumab-IgG4/κ

    Plasmid
    #61887
    Purpose
    Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61887-plasmid-map-sequence-id-101186

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Showing: 2641 - 2680 of 173176 results