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Plasmid#86390PurposeExpression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoterDepositorInsertD. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
UseStap-seq spike in vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD284
Plasmid#66825PurposeTagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized TagRFP-TExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-mEYFP
Plasmid#87666Purposeretroviral vector encoding cre dependent expression of membrane EYFPDepositorInsertMem-EYFP
UseRetroviralTagspalmitylationExpressionMutationPromoterCAGAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
RV-Syn-Dio-GFP
Plasmid#87673Purposeretroviral vector encoding cre dependent expression of GFPDepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterSynapsinAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNM3746
Plasmid#154109PurposeloxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-mTdt
Plasmid#87665Purposeretroviral vector encoding cre dependent expression of membrane TdtomatoDepositorInsertMem-Tdtomato
UseRetroviralTagsExpressionMutationPromoterCAGAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
RV-Syn-Dio-mTdt
Plasmid#87667Purposeretroviral vector encoding cre dependent expression of membrane TdtomatoDepositorInsertMem-Tdtomato
UseRetroviralTagspalmitylationExpressionMutationPromoterSynapsinAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-kikume
Plasmid#87663Purposeretroviral vector encoding cre-dependent expression of kikumeDepositorInsertkikume
UseRetroviralTagsExpressionMutationPromoterCAGAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQlox66R
Plasmid#135657PurposeClostridial vector, encoding a targetron carrying a lox66 site in the reverse (antisense) orientation. Retarget intron to insert lox66 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQadd1R
Plasmid#135661PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQlox71R
Plasmid#135656PurposeClostridial vector, encoding a targetron carrying a lox71 site in the reverse (antisense) orientation. Retarget intron to insert lox71 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQlox71F
Plasmid#135655PurposeClostridial vector, encoding a targetron carrying a lox71 site in the forward (sense) orientation. Retarget intron to insert lox71 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQlox66F
Plasmid#135658PurposeClostridial vector, encoding a targetron carrying a lox66 site in the forward (sense) orientation. Retarget intron to insert lox66 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA
Plasmid#87572PurposePV4 gRNA entry vector for CRISPR decoderDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA
Plasmid#89056PurposePV3 gRNA entry vector for CRISPR decoderDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX_305_Ovalbumin
Plasmid#184924PurposeLentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.DepositorInsertOvalbumin (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationOur vector has an A188T mutation that does not im…PromoterPGKAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetON-CD4
Plasmid#217537PurposeAAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptorDepositorArticleInsertCD4 receptor (CD4 Human)
UseAAVTagsHA epitopeExpressionMammalianMutationPromoterTetOperon (TetO)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-1x-ccdB-6stop
Plasmid#203519PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_repA1
Plasmid#211910PurposeReplicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_repA3
Plasmid#211912PurposeReplicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only