We narrowed to 10,294 results for: otos
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Plasmid#108112PurposeLentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchange cytosine 333 to alanine (silent mutation)PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF5B*-strep
Plasmid#120164PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidinDepositorInsertkinesin family member 5B (Kif5b Mouse)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF5B: 1-555 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-FCHO1
Plasmid#196910Purposevisualization of clathrin-coaed vesicle formation in living mammalian cellsDepositorAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221E
Plasmid#108108PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
ExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…Available SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1
Plasmid#22027PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
Strep-KIFC1*-mCh
Plasmid#120169PurposeExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)DepositorInsertkinesin family member C1 (KIFC1 Human)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIFC1: 125-673 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
opto-E-cad GFP
Plasmid#203327PurposeExpresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cellsDepositorInsertPhotoswitchable - E-cadherin with GFP tag (CDH1 Human)
TagsGFPExpressionMammalianMutationAddition of Aslov2 domain at T133 of human E-cadh…Available SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiOn*-CAG∞RFP
Plasmid#154019PurposeVector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
ExpressionMammalianMutationAll TTAA in vector backbone were replaced by sile…PromoterCAGAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-KIF5B*-strep
Plasmid#120170PurposeExpresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidinDepositorInsertkinesin family member 5B (Kif5b Mouse)
TagsHA and StreptavidinExpressionMammalianMutationTruncated KIF5B: 1-555PromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1
Plasmid#22024PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-C (TRP1)
Plasmid#177795PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneTagsDHFR F[1,2]ExpressionYeastPromoterADH1Available SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-MIR302-7guides-PGK-Puro
Plasmid#201960PurposeEBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassetteDepositorInsertMIR302-7g-PGK-Puro
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Strep-KIFC1*-HA
Plasmid#120171PurposeExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tagDepositorInsertkinesin family member C1 (KIFC1 Human)
TagsHA and StreptavidinExpressionMammalianMutationTruncated KIFC1: 125-673 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF1A*-strep
Plasmid#120165PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidinDepositorInsertkinesin family member 1A (Kif1a Mouse)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF1A: 1-405 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169
Plasmid#178528PurposeExpresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cellsDepositorInsertrtTA, SspB-mScarlet-I-MYPT (1-169aa)
ExpressionMammalianPromoterCAG, Tet responsive elementAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1
Plasmid#22007PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
LAMP-mChF-AIP
Plasmid#61524PurposeExpression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIPDepositorInsertlysosomal-associated membrane protein 1 (LAMP1 Human)
TagsAIP and mCherryExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-GRAB-HTR6-cilia
Plasmid#189615PurposeExpresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoterDepositorInsertHTR6_GRAB
UseAAVPromoterSyn1Available SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGM32DEST
Plasmid#74747PurposeEncodes for the QF-GR recombinant gene, splice linker, and mCherry reporter geneDepositorInsertsTagsFusion of the Neurospora crassa QF activator to t…ExpressionWormPromoterNo promoterAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP
Plasmid#61527PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIPDepositorInsertFK506 binding protein 1A (FKBP1A Human)
TagsAIP and mCherryExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(wt)
Plasmid#107614PurposeN-terminal half of π-EB1 with wild-type LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
TagsA. sativa phototropin 1 LOV2 domain and GCN4 leuc…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP(TA)
Plasmid#61528PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TA mutation)DepositorInsertFK506 binding protein 1A (FKBP1A Human)
TagsAIP with TA mutation (see comments) and mCherryExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-Cx43-GFP
Plasmid#176860PurposeExpresses Cx43 fused with GFP at the C-terminus in astrocytesDepositorAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-N (TRP1)
Plasmid#177797PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneTagsDHFR F[1,2]ExpressionYeastPromoterADH1Available SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Az
Plasmid#197101PurposeTo express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanineDepositorInsertTyrRS variant, amber suppressor tRNA, kanamycin resistance gene
ExpressionBacterialMutationThr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-GG-OCT4-1-5-PGK-Puro
Plasmid#102893PurposePiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.DepositorAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
Plasmid#173862PurposeMammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)DepositorInsertAktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
TagsC-terminal CAAX-box and mCherryExpressionMammalianPromoterCAGAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-Venus
Plasmid#139502PurposeExpresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
TagsVenus tag following 3x Ala linkerExpressionMammalianAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only