We narrowed to 3,240 results for: Streptococcus

Showing: 1341 - 1360 of 3240 results

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PX379 Legionella pneumophila str. Paris Cas9

Plasmid

#68316

Purpose

Legionella pneumophila str. Paris Cas9

Depositor

Feng Zhang

Insert

LpCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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PX391 Sphaerochaeta globus str. Buddy Cas9

Plasmid

#68328

Purpose

Sphaerochaeta globus str. Buddy Cas9

Depositor

Feng Zhang

Insert

SgCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

Plasmid

#133340

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

Depositor

Michael Laub

Publication

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site1 (RTW443)

Plasmid

#160136

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pS848∙CsR

Plasmid

#199240

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Gm resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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AAEL007097-Cas9

Plasmid

#100705

Purpose

The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Publication

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL007097-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL007097 and Opie2

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAGT6471

Plasmid

#219428

Purpose

Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)

Depositor

Tom Schreiber

Publication

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

AGGT_NLS-SpCas9i-NLS(no stop)_TTCG

Use

Moclo compatible level 0 module

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6a

Plasmid

#215819

Purpose

Express CBE6a (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6a-SpCas9-2xUGI (cas9 )

Expression

Mammalian

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAN053

Plasmid

#220047

Purpose

Mammalian expression of type III-A crRNA from Streptococcus thermophilus within 5`UTR of mCherry transcript

Depositor

Blake Wiedenheft

Publication

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA-mCherry

Expression

Mammalian

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAN102

Plasmid

#220045

Purpose

Mammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Publication

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA; Cas6

Expression

Mammalian

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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PsaA

Plasmid

#159997

Purpose

Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

PsaA (19-309)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Spy0469

Plasmid

#159996

Purpose

Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

Spy0469 (24-389)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d

Plasmid

#215822

Purpose

Express CBE6d (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI (cas9 )

Expression

Mammalian

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6c

Plasmid

#215821

Purpose

Express CBE6c (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI (cas9 )

Expression

Mammalian

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

Plasmid

#132667

Purpose

Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-ODD-T2A-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Promoter

4xHRE_YB TATA

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-SpCas9-HF1 (without sgRNA)

Plasmid

#92102

Purpose

Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Publication

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

Cbh

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAGM4723:TpCC_Urease

Plasmid

#85982

Purpose

Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

Depositor

Thomas Mock

Publication

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Inserts

Cas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2

Use

CRISPR; Diatom (t. pseudonana)

Tags

NLS and YFP

Promoter

FCP and U6

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pNORg504

Plasmid

#249345

Purpose

Mediterraneibacter gnavus CRISPR-cas9 mutagenesis plasmid

Depositor

Nozomu Obana

Publication

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Inserts

sgRNA - cas9
homology arm for srtB4 deletion

Use

E. coli - r. gnavus shuttle vector

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pET-Octo_VirD2_NLS-Cas9-6xHis

Plasmid

#232296

Purpose

Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

Depositor

Hee-Sik Kim

Publication

Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

Octo_VirD2_NLS-Cas9-6xHis

Use

CRISPR

Tags

6xHis and Octopine-type VirD2-derived NLS

Expression

Bacterial

Promoter

T7

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse cGAS #1-gRNA-Cas9-Puro

Plasmid

#186890

Purpose

gRNA targeting mouse cGAS (with Cas9 insert)

Depositor

Jonathan Kagan

Publication

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Cgas Mouse)

Use

Lentiviral

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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