We narrowed to 3,122 results for: rho

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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA on ChR2

Promoter

CBA

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-flex-oChIEF-citrine

Plasmid

#50973

Purpose

flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

inverted and inserted between two lox sites

Promoter

human synapsin promoter

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

Plasmid

#172910

Purpose

Mammalian expression of voltage sensor; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2-ST

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDD112

Plasmid

#119893

Purpose

pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-mDia1FH2deltaN

Plasmid

#25419

Depositor

Arthur Alberts

Article

Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

Insert

diaph1 (Diaph1 Mouse)

Tags

YFP A206K

Expression

Mammalian

Mutation

mDia1 FH2∆N2 sequence (encodes amino acids 771-11…

Available Since

July 20, 2010

Availability

Academic Institutions and Nonprofits only
AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA on ChR2

Promoter

CBA

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-flex-oChIEF-citrine

Plasmid

#50973

Purpose

flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

inverted and inserted between two lox sites

Promoter

human synapsin promoter

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD112

Plasmid

#119893

Purpose

pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-mDia1FH2deltaN

Plasmid

#25419

Depositor

Arthur Alberts

Article

Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

Insert

diaph1 (Diaph1 Mouse)

Tags

YFP A206K

Expression

Mammalian

Mutation

mDia1 FH2∆N2 sequence (encodes amino acids 771-11…

Available Since

July 20, 2010

Availability

Academic Institutions and Nonprofits only
pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

Plasmid

#125256

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

Depositor

Roger D. Kamm

Article

Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

Insert

hChR2(H134R)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF1a-forward

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183523

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

I146M/G174S

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

Plasmid

#125256

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

Depositor

Roger D. Kamm

Article

Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

Insert

hChR2(H134R)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF1a-forward

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-rc[Chronos-GFP]

Plasmid

#59056

Purpose

Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-Neo-PTPRT (WT)

Plasmid

#16630

Depositor

Bert Vogelstein

Article

Wang et al Science. 2004 May 21. 304(5674):1164-6.

Insert

PTPRT (PTPRT Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII-ChrimsonR-tdTomato

Plasmid

#99231

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

CaMKII

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2

Plasmid

#202761

Purpose

Encodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harbor

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

NEUROG2-p2A-ATOH7-p2A-ISL1-p2A-POU4F2 (NEUROG2, ATOH7, ISL1, POU4F2 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-rc[Chronos-GFP]

Plasmid

#59056

Purpose

Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pSLIK DN Rac1

Plasmid

#84642

Purpose

Lentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Dominant Negative

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only
pCI-Neo-PTPRT (WT)

Plasmid

#16630

Depositor

Bert Vogelstein

Article

Wang et al Science. 2004 May 21. 304(5674):1164-6.

Insert

PTPRT (PTPRT Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII-ChrimsonR-tdTomato

Plasmid

#99231

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

CaMKII

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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