We narrowed to 213 results for: EcoRI

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pIM1708-surT-PsurP-agaN-pNW33N

Plasmid

#44009

Depositor

Ichiro Matsumura

Article

Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Insert

surT-PsurP-agaN

Expression

Bacterial

Mutation

Deleted EcoRI site (synonymous change, C to T at …

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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MXS_MCS2

Plasmid

#62396

Purpose

MXS_chaining vector with MCS2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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HMEKc-srcM

Plasmid

#1107

Depositor

Susan Lindquist

Article

Xu et al Proc Natl Acad Sci U S A 1999 Jan 5;96(1

Insert

c-src

Expression

Yeast

Mutation

MET3 promoter inserted in EcoRI and BamHI of pRS3…

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pLenti-STAT3-linker-TagGFP2

Plasmid

#121427

Purpose

WT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

STAT3-linker-TagGFP2

Tags

TagGFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGBT9-tepsin

Plasmid

#196647

Purpose

Expression of GAL4 DNA-binding domain (BD)-tepsin fusion protein

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

tepsin (TEPSIN Human)

Use

Yeast two-hybrid vector

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

silent substitution in codon 199 to eliminate int…

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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KBB215

Plasmid

#185068

Purpose

SST::WAK2 EcoRI/ScaI fragment (secretion confirmed)

Depositor

Ken Belanger

Article

Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

Insert

WAK2

Expression

Yeast

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEIIIA

Plasmid

#14059

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIA (Fn1 Rat)

Expression

Mammalian

Mutation

EcoRI linkers added to TaqI to HinfI fragment

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pJM614-TRA-2::GFP

Plasmid

#27128

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 GFP (tra-2 Nematode)

Tags

GFP

Expression

Worm

Mutation

replaced stop codon with AgeI and EcoRI added GFP…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pEIIIB

Plasmid

#14060

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIB (Fn1 Rat)

Expression

Mammalian

Mutation

EcoRI linkers were attached to HindIII - HinfI fr…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
YF-Cdc42(CA)

Plasmid

#20152

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Cdc42(CA) (CDC42 Human)

Expression

Mammalian

Mutation

Cdc42H was inserted into the multiple cloning sit…

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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GST-MAGE-2-429

Plasmid

#89129

Purpose

bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pLpC-Reg-L-IFP-F[2]+Venus (mammalian)

Plasmid

#52903

Purpose

Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Reg-L-IFP-F[2]+Venus

Use

Retroviral

Tags

L-IFP-F[2]

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pPV230.13

Plasmid

#14902

Depositor

James Lok

Article

Li et al Int J Parasitol. 2006 May 31. 36(6):671-

Insert

Strongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region

Expression

Worm

Mutation

Ss era-1 promoter region as a HindIII-PstI fragme…

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)

Plasmid

#40341

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

Blimp1 promoter (mutated) (PRDM1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Two AP-1 sites at -1813 and -1647 replaced with B…

Promoter

Blimp1

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Flag-TUBG2

Plasmid

#229855

Purpose

TUBG2

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

Insert

TUBG2 (TUBG2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Removal of the internal TUBG2-EcoRI site by chang…

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMD19T-psba1-TetR-PL22-dCas9-SpR

Plasmid

#73223

Purpose

Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PL22

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSG5-ERRbeta2

Plasmid

#52186

Purpose

Expresses human ERRbeta2 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only