Addgene

We narrowed to 19,127 results for: KIN

Showing: 121 - 140 of 19127 results

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pCMV-mKO2-Parkin

Plasmid

#213544

Purpose

Transient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact

Depositor

Lani Wu, Steven Altschuler

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Tags

mKO2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Parkin(E16A)

Plasmid

#213546

Purpose

Transient mammalian expresssion of E16A Parkin

Depositor

Lani Wu, Steven Altschuler

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Tags

Expression

Mammalian

Mutation

E16A mutation in Parkin

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Mss4-Kina

Plasmid

#202739

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:S. cerevisiae Mss4 (377-756) (MSS4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4D636K-Kina

Plasmid

#202725

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4-Kina

Plasmid

#202724

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756) (MSS4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Mss4D636K-Kina

Plasmid

#202740

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:S. cerevisiae Mss4 (377-756)(D636K) (MSS4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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TetO::EGFP-C-Meikin

Plasmid

#174706

Purpose

Doxycycline inducible expression of human C-Meikin Separase cleavage fragment

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Tags

EGFP

Expression

Mammalian

Mutation

155-373

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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myc-Miro2 (lacking TM)

Plasmid

#127614

Purpose

Human Miro2 with the transmembrane deleted

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Miro2 (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Transmembrane deletion

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHD-dsRed-attP_dCrk_flankingHRs

Plasmid

#131141

Purpose

Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Inserts

Crk Upstream Homology Region (Crk Fly)
Crk Downstream Homology Region (Crk Fly)

Use

CRISPR and Cre/Lox; Homology directed repair temp…

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP KInd2 S159E

Plasmid

#105311

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159E

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S666A

Plasmid

#105308

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S666A

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP KInd2 S159A

Plasmid

#105306

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159A

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector_blocking 2

Plasmid

#99317

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-2x-Rhotekin-GBD

Plasmid

#214142

Purpose

Rho activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xRhotekin-GBD (Rtkn Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin-A92mKO2

Plasmid

#213559

Purpose

Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

Depositor

Lani Wu, Steven Altschuler

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ATR Kinase dead

Plasmid

#31612

Depositor

Aziz Sancar

Article

Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

Insert

ATR (ATR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K2327R (kinase dead)

Promoter

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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myc mTOR kinase dead

Plasmid

#8482

Depositor

David Sabatini

Insert

mTOR (Mtor Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

D2357E V2364I

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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YFP-Parkin-IRES-zeo

Plasmid

#61728

Purpose

Expression of human Parkin fused to eYFP

Depositor

Stephen Tait, Douglas Green

Article

Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

Insert

Parkin (PRKN Human)

Use

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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TagBFP2-FKBP-PIP5K1C_Kina-HDD316K

Plasmid

#202742

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:FKBP1A (3-108) : [GGSA]4GG : PIP5K1C(79-366) (D101R / R304D / D316K) (PIP5K1C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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