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Showing: 121 - 140 of 279 results

  5. pLM-AMA18.0 dCas9-VPR

    Plasmid
    #138945
    Purpose
    Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138945-plasmid-map-sequence-id-366628

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  9. pMSCV-LTR-dCas9-p65AD-BFP

    Plasmid
    #46913
    Purpose
    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP
    Depositor
    Jonathan Weissman, Stanley Qi
    Article
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Inserts
    dCas9-p65AD-BFP fusion
    Puromycin resistance
    Use
    CRISPR and Retroviral
    Tags
    2xNLS, BFP, and VP64 domain
    Expression
    Mammalian
    Mutation
    Promoter
    LTR and PGK
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    46913-plasmid-map-sequence-id-66611

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  10. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84244-plasmid-map-sequence-id-160887

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  11. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

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  12. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

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  14. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  17. pMLM3705

    Plasmid
    #47754
    Purpose
    Expresses mammalian cell codon-optimized dCas9-VP64
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Insert
    codon optimized dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9 (catalytically i…
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47754-plasmid-map-sequence-id-68222

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  18. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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  19. pBUN6A11

    Plasmid
    #50579
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50579-plasmid-map-sequence-id-75626

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  20. pYPQ173

    Plasmid
    #99907
    Purpose
    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
    Depositor
    Yiping Qi
    Article
    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(
    Insert
    pco-dCas9-VP64-T2A-MS2-VP64
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    99907-plasmid-map-sequence-id-210432

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Showing: 121 - 140 of 279 results