We narrowed to 7,375 results for: ef1a

1. crTet in pSLQ8453

   Plasmid

   #183965

   Purpose

   U6-crTet-EF1a-Puro-T2A-BFP-WPRE

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   crTet
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MCP-3x9aaTAD

   Plasmid

   #210707

   Purpose

   This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-puro-N-Flag-Snrnp40

   Plasmid

   #134253

   Purpose

   Lentivector encoding Flag-tagged Snrnp40

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   Snrp40 (Snrnp40 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-iRFP-STOP-ReNL

   Plasmid

   #113965

   Purpose

   Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)

   Depositor

   Jordan Green

   Article

   Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

   Inserts

   iRFP670
   ReNL
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV - SV40-PolyA - SV40-PolyA and EF1A

   Available Since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti NbALFA-mNeonGreen puro

   Plasmid

   #193973

   Purpose

   lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, puromycin resistance

   Depositor

   Raphael Gaudin

   Insert

   NbALFA-mNeonGreen
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti NbALFA-miRFP680 hygro

   Plasmid

   #193969

   Purpose

   lentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, hygromycin resistance

   Depositor

   Raphael Gaudin

   Insert

   NbALFA-miRFP680
   

   Use

   Lentiviral

   Tags

   miRFP680

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti NbALFA-miRFP680 puro

   Plasmid

   #193972

   Purpose

   lentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, puromycin resistance

   Depositor

   Raphael Gaudin

   Insert

   NbALFA-miRFP680
   

   Use

   Lentiviral

   Tags

   miRFP680

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti NbALFA-mNeonGreen hygro

   Plasmid

   #193974

   Purpose

   lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, hygromycin resistance

   Depositor

   Raphael Gaudin

   Insert

   NbALFA-mNeonGreen
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. piggyBAC-U6-GFP-WPRE

   Plasmid

   #200030

   Purpose

   piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

   Depositor

   Jay Shendure

   Type

   Empty backbone

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROP-seq-opti-dsRed

    Plasmid

    #201999

    Purpose

    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.

    Depositor

    Daniel Geschwind

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-P-TAG (pGS594)

    Plasmid

    #160039

    Purpose

    pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Insert

    CP450TMD-APEX2-3xHA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY0551-Luciferase RADARS

    Plasmid

    #182538

    Purpose

    Mammalian cell expression plasmid of Gaussia luciferase RADARS in a dual luciferase expression back bone with Cypridina luciferase as the control gene.

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Insert

    RADARS Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. TK4-EGFP

    Plasmid

    #239047

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

    Plasmid

    #219828

    Purpose

    Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLV Rnf121-V5

    Plasmid

    #175130

    Purpose

    Lentiviral expression of mouse Rnf121-V5

    Depositor

    Larry Gerace

    Insert

    Rnf121 (Rnf121 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-RfxCas13d-Blast-BFP

    Plasmid

    #196726

    Purpose

    Sortable and selectable RfxCas13d.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV Nup62-V5

    Plasmid

    #175116

    Purpose

    Lentiviral expression of mouse Nup62-V5

    Depositor

    Larry Gerace

    Insert

    Nup62 (Nup62 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid

    #171939

    Purpose

    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein

    Depositor

    David Berson

    Insert

    APEX2-eGFPf
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV Tor1a-V5

    Plasmid

    #175135

    Purpose

    Lentiviral expression of Tor1a-Myc

    Depositor

    Larry Gerace

    Insert

    Tor1a (Tor1a Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV V5-Mospd1

    Plasmid

    #175132

    Purpose

    Lentiviral expression of V5-tagged mouse Mospd1

    Depositor

    Larry Gerace

    Insert

    Mospd1 (Mospd1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV Tyw1-V5

    Plasmid

    #175131

    Purpose

    Lentiviral expression of mouse Tyw1-V5

    Depositor

    Larry Gerace

    Insert

    Tyw1 (Tyw1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid

    #229498

    Purpose

    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    MYC 5'Untranslated region + destabilized GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAVS1-iSAM

    Plasmid

    #211495

    Purpose

    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

    Depositor

    Antonella Fidanza, Lesley Forrester

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-mCherry Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, NA, Splicing acceptor, and TREGV promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lentiMPH v2 (Neo)

    Plasmid

    #92065

    Purpose

    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Insert

    MS2-P65-HSF1_2A_Neo (HSF1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available Since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Has Service

    AAV1

    Insert

    mKate2-PDE4DCatL (PDE4D Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAC150-KEIMA-VAPA

    Plasmid

    #212096

    Purpose

    Fluorescent reporter for VAPA clearance to acidic lysosomes

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    VAPA (VAPA Human)
    

    Use

    Piggybac

    Tags

    mKeima

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo

    Plasmid

    #222636

    Purpose

    Ezrin activation reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    miRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-puro-N-Flag-h-SNRNP40

    Plasmid

    #134254

    Purpose

    Lentivector encoding Flag-tagged human SNRNP40

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    SNRP40 (SNRNP40 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid

    #127668

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and hU6

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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