We narrowed to 7,375 results for: ef1a

1. pLV-Hyg-Snrnp40-CRISPR-resistant

   Plasmid

   #134251

   Purpose

   Lentivector encoding CRISPR-resistant Snrnp40

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   Snrp40 (Snrnp40 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mutated coding sequence “gataactatgcgacgttgaa” to…

   Promoter

   EF1a

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LADL Bridge + Promoter Only Target

   Plasmid

   #127669

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and hU6

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo

   Plasmid

   #222637

   Purpose

   Ezrin activation reporter, T567 phosphorylation deficient

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   miRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mutation

   T567A

   Promoter

   EF1a

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV Tmx1-V5

   Plasmid

   #175138

   Purpose

   Lentiviral expression of V5-tagged mouse Tmx1

   Depositor

   Larry Gerace

   Insert

   Tmx1 (Tmx1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-Puro_hCOL4A4

   Plasmid

   #198099

   Purpose

   lentiviral expression of hCOL4A4 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A4 (COL4A4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

   Plasmid

   #219826

   Purpose

   Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 29, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLV V5-Nesprin-3

   Plasmid

   #175136

   Purpose

   Lentiviral expression of V5-tagged mouse Syne3

   Depositor

   Larry Gerace

   Insert

   Syne3 (Syne3 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV V5-Sun2

   Plasmid

   #175114

   Purpose

   Lentiviral expression of V5-tagged mouse Sun2

   Depositor

   Larry Gerace

   Insert

   Sun2 (Sun2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi-Pur-AktAR2

   Plasmid

   #125195

   Purpose

   SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter

   Depositor

   Eric Davis

   Insert

   AktAR2
   

   Use

   Transposon

   Tags

   6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV Tmem9b-V5

    Plasmid

    #175152

    Purpose

    Lentiviral expression of mouse Tmem9b-V5

    Depositor

    Larry Gerace

    Insert

    Tmem9b (Tmem9b Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV Ndc1-V5

    Plasmid

    #175115

    Purpose

    Lentiviral expression of mouse Ndc1-V5

    Depositor

    Larry Gerace

    Insert

    Ndc1 (Ndc1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV Tor1a-Myc

    Plasmid

    #175134

    Purpose

    Lentiviral expression of Myc-tagged mouse Tor1a

    Depositor

    Larry Gerace

    Insert

    Tor1a (Tor1a Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV V5-Sun1

    Plasmid

    #175113

    Purpose

    Lentiviral expression of V5-tagged mouse Sun1

    Depositor

    Larry Gerace

    Insert

    Sun1 (Sun1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV Nup88-V5

    Plasmid

    #175117

    Purpose

    Lentiviral expression of mouse Nup88-V5

    Depositor

    Larry Gerace

    Insert

    Nup88 (Nup88 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV Rbmxl1-V5

    Plasmid

    #175124

    Purpose

    Lentiviral expression of mouse Rbmxl1-V5

    Depositor

    Larry Gerace

    Insert

    Rbmxl1 (Rbmxl1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-dCas9-VP64-BFP

    Plasmid

    #196711

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-VP64-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-UCOE-dCas9-BFP-VP64

    Plasmid

    #196717

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-Puro_hCOL4A4-3FLAG

    Plasmid

    #198101

    Purpose

    lentiviral expression of hCOL4A4 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A4 (COL4A4 Human)
    

    Use

    Lentiviral

    Tags

    3FLAG

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-Hygro_hCOL4A3

    Plasmid

    #198098

    Purpose

    lentiviral expression of hCOL4A3 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A3 (COL4A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ELL3 Streptag

    Plasmid

    #127282

    Purpose

    Expresses mouse ELL3 with streptactin tag; does it degrade Myc?

    Depositor

    Christine Milcarek

    Insert

    ELL3 (NM_145973) (ELL3 Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid

    #125206

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter

    Depositor

    Eric Davis

    Insert

    LPAR_AKAR-WT
    

    Use

    Transposon

    Tags

    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLV V5-Senp2

    Plasmid

    #175118

    Purpose

    Lentiviral expression of V5-tagged mouse Senp2

    Depositor

    Larry Gerace

    Insert

    Senp2 (Senp2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. gTBEv3

    Plasmid

    #220619

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    hUNG2-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. gGBEv6.3-SpCas9

    Plasmid

    #202629

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpCas9(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

    Promoter

    hU6, EF1a, CBh

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLV-puro-N-3HA-SREBP1-C-Flag

    Plasmid

    #134286

    Purpose

    Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Lentiviral

    Tags

    3x HA and Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    EF1a

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. gGBEv6.3-SpG

    Plasmid

    #202630

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpG(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

    Promoter

    hU6, EF1a, CBh

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. gCBEv2

    Plasmid

    #220617

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    hUNG2-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAAV-nEF Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55647

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Short Ef1a

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. LentiU6-LacZ-GFP-Puro (BB)

    Plasmid

    #170459

    Purpose

    For cloning gRNA block

    Depositor

    Yonglun Luo

    Article

    Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

    Insert

    hU6-BB-LacZ and EF1A-EGFP-2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 for gRNA and EF1A for EGFP-2A-Puro

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

    Plasmid

    #229826

    Purpose

    Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet-I
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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