Addgene

We narrowed to 7,088 results for: ef1a

Showing: 2101 - 2130 of 7088 results

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pLV_Stargazin-mTurquoise2-iLID

Plasmid

#161001

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Stargazin-mTurquoise2-iLID

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Baz1a.1-GFP-WPRE

Plasmid

#211490

Purpose

expresses GFP under artificial promoter Baz1a

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_ADRB2-mTurquoise2-iLID

Plasmid

#161002

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

ADRB2-mTurquoise2-iLID

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EgI-BlastR

Plasmid

#207176

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Agmat.1-GFP-WPRE

Plasmid

#211489

Purpose

expresses GFP under artificial promoter Agmat

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_mTurquoise2-iLID-CAAX

Plasmid

#160999

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

mTurquoise2-iLID-CAAX

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EgI-NeoR

Plasmid

#207175

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 WT

Plasmid

#61735

Purpose

Expresses WT human NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

wild type

Promoter

EF1a

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLOX-EWgfp

Plasmid

#12242

Depositor

Didier Trono

Article

Salmon et al Blood. 2000 Nov 15. 96(10):3392-8.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

EF1a promoter is upstream of GFP. LoxP in 3'…

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pEBB-TB

Plasmid

#36099

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Use

Tags

BT-tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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p8RwB

Plasmid

#48733

Purpose

dsRed plasmid

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

dsRed-Express

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128F

Plasmid

#61734

Purpose

Expresses human Y128F NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

Y128F

Promoter

Ef1a

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pL_FR_Hygro

Plasmid

#127171

Purpose

Frameshift reporter (control plasmid for screening)

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

Frameshift Reporter

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS

Plasmid

#163083

Purpose

Donor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENs

Depositor

Lacra Bintu

Article

Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

Insert

TagRFP-T

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128V

Plasmid

#61733

Purpose

Expresses human Y128V NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

Y128V

Promoter

Ef1a

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-MVPH

Plasmid

#235600

Purpose

Expresses the MS2-VP64-P65-HSF1 fusion protein

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

MS2-VP64-P65-HSF1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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Zdk3-VPR

Plasmid

#231572

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VPR, Ef1a driven

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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LEAPVW

Plasmid

#205671

Purpose

shRNAmir backbone for RNA interference, with Venus reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-1α-p37-mClover2

Plasmid

#225500

Purpose

Expression of mClover2-tagged p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral

Tags

mClover2

Expression

Mammalian

Mutation

no

Promoter

EF1A

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAF201

Plasmid

#222376

Purpose

Recombinant Tasmanian devil IgG antibody (1F8) that binds to Tasmanian devil PDL1

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAF200

Plasmid

#222375

Purpose

Recombinant Tasmanian devil IgG antibody (3D5) that binds to 6xHis at the C-terminus of proteins

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

Plasmid

#219823

Purpose

EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

Depositor

Philipp Maass

Insert

RfxCas13d

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a core promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LEAPAW

Plasmid

#205670

Purpose

shRNAmir backbone for RNA interference, with Ametrine reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LEAPTW

Plasmid

#205668

Purpose

shRNAmir backbone for RNA interference, with mTagBFP2 reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LEAPOW

Plasmid

#205672

Purpose

shRNAmir backbone for RNA interference, with mOrange reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LEAPZW

Plasmid

#205667

Purpose

shRNAmir backbone for RNA interference, with Azurite reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LEAPGW

Plasmid

#205669

Purpose

shRNAmir backbone for RNA interference, with GFP reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LEAPEW

Plasmid

#205674

Purpose

shRNAmir backbone for RNA interference, with E2-Crimson reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LEAPPW

Plasmid

#205675

Purpose

shRNAmir backbone for RNA interference, with Puromycin selection

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge