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Showing: 2801 - 2840 of 3064 results

  1. pCLV3Cas-LT1

    Plasmid
    #210549
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
    Use
    Tags
    Expression
    Plant
    Mutation
    2bp insertion after AAVS1 target sequence that di…
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    210549-plasmid-map-sequence-id-426236

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  2. pPPEM

    Plasmid
    #162467
    Purpose
    Destination Expression vector for monocot plant primer editing
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Insert
    Cas9H840A
    Use
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Promoter
    maize Ubi
    Available since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    162467-plasmid-map-sequence-id-350385

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  4. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65769-plasmid-map-sequence-id-112659

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  7. p CIneo-RL-Let7-3xBulgeB-mut

    Plasmid
    #115369
    Purpose
    Expression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTR
    Depositor
    Ramesh Pillai, Witold Filipowicz
    Article
    Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.
    Insert
    Renilla Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115369-plasmid-map-sequence-id-223055

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  8. BPK2301

    Plasmid
    #65778
    Purpose
    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    St1Cas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65778-plasmid-map-sequence-id-112652

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  16. pDN-D2irTN2AG5kwh

    Plasmid
    #128253
    Purpose
    Mammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.
    Depositor
    Gabor Balazsi
    Article
    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:
    Insert
    Beta Globin Intron::hTetR::P2A::EGFP
    Use
    Synthetic Biology; Flp-in expression vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV D2ir promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    128253-plasmid-map-sequence-id-248939

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  19. pSBL_DK198

    Plasmid
    #156425
    Purpose
    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Use
    Tags
    sfGFP-His
    Expression
    Insect
    Mutation
    aa 42-275
    Promoter
    Polyhedrin
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156425-plasmid-map-sequence-id-307748

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  32. pCLV3Cas-LT2

    Plasmid
    #210550
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
    Use
    Tags
    Expression
    Plant
    Mutation
    1bp insertion after AAVS1 target sequence that di…
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    210550-plasmid-map-sequence-id-426233

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  34. pLP-pTac-himar-apraR

    Plasmid
    #199995
    Purpose
    Landing Pad with T7 RNAP circuit variant T15. pIP1443 seed promoter. pTac-himar transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. For more information look below.
    Depositor
    Farren Isaacs
    Article
    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.
    Insert
    Landing Pads
    Use
    Tags
    Expression
    Bacterial
    Mutation
    himar frameshift
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    199995-plasmid-map-sequence-id-423477

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  36. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Article
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

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  37. pYPQ265N2

    Plasmid
    #174001
    Purpose
    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    rAPOBEC1-zCas9(D10A)-SpRY-UNG
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174001-plasmid-map-sequence-id-343426

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  38. pYPQ265O2

    Plasmid
    #174002
    Purpose
    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174002-plasmid-map-sequence-id-343429

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  39. pYPQ265L2

    Plasmid
    #174000
    Purpose
    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174000-plasmid-map-sequence-id-343423

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Showing: 2801 - 2840 of 3064 results