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Showing: 3551 - 3600 of 6939 results

  2. pMS_366

    Plasmid
    #182131
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation
    Depositor
    George Church
    Article
    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Use
    Tags
    Expression
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Promoter
    Available since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    182131-plasmid-map-sequence-id-355782

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  7. pOPS0384

    Plasmid
    #133234
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133234-plasmid-map-sequence-id-287535

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  21. pLenti-EF1a-MTS-FLAG-LOV*

    Plasmid
    #202208
    Purpose
    Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells
    Depositor
    Thomas Muir
    Article
    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12
    Insert
    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
    Use
    Lentiviral
    Tags
    FLAG and LOV*
    Expression
    Mutation
    Promoter
    Ef1a
    Available since
    Aug. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    202208-plasmid-map-sequence-id-402886

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  23. RNA polymerase beta prime subunit FLAG

    Plasmid
    #102337
    Purpose
    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.
    Depositor
    Erin O'Shea
    Article
    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1
    Insert
    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    Use
    Tags
    3x FLAG with 3X GSGS linker
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102337-plasmid-map-sequence-id-197499

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  25. pOPS0385

    Plasmid
    #133235
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133235-plasmid-map-sequence-id-287549

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  26. pOPS0386

    Plasmid
    #133236
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133236-plasmid-map-sequence-id-287551

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  27. pOPS0383

    Plasmid
    #133233
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133233-plasmid-map-sequence-id-287536

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  29. pCDNA3_PKA-CαN (K29,30A)-mVenus

    Plasmid
    #168485
    Purpose
    Mammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168485-plasmid-map-sequence-id-332538

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  30. pSLiP-G1

    Plasmid
    #188963
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA: atcggtcgcattgttttccactagg
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    188963-plasmid-map-sequence-id-371923

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  33. PumC

    Plasmid
    #87321
    Purpose
    PumHD w/each unit mutated to bind C (as in Fig. 1C; unit 6 is the Pumby for C)
    Depositor
    Edward Boyden
    Article
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Insert
    Pumilio protein mutant (PUM1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    all 8 units mutated to bind C
    Promoter
    UBC
    Available since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    87321-plasmid-map-sequence-id-181387

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  39. pBW300ParsR-Amp32T

    Plasmid
    #78652
    Purpose
    pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-
    Insert
    araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    see insert
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  44. MC177VSG221RNAi

    Plasmid
    #59730
    Purpose
    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.
    Depositor
    Gloria Rudenko
    Article
    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10
    Insert
    VSG221 RNAi fragment
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    opposing T7 promoters
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    59730-plasmid-map-sequence-id-93746

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Showing: 3551 - 3600 of 6939 results