We narrowed to 13,592 results for: crispr cas9

1. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

   Plasmid

   #59791

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized VP64 dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

   Plasmid

   #164038

   Purpose

   Expression of dCas9-14xGFP11 in mammalian cells

   Depositor

   Baohui Chen

   Article

   Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

   Insert

   dCas9-14xGFP11
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   dSV40

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

   Plasmid

   #99248

   Purpose

   single-vector system with EGFP reporter for use in CROPseq knockout screens

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-Tet-Cas9-BFP-PGK-Blast

   Plasmid

   #196720

   Purpose

   Tet-inducible expression of Cas9-T2A-TagBFP

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Expression

   Mutation

   Promoter

   TRE, PGK

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

   Plasmid

   #196722

   Purpose

   Tet-inducible expression of Cas9-T2A-miRFP670nano

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-miRFP670nano
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Expression

   Mutation

   Promoter

   TRE, PGK

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

   Plasmid

   #160618

   Purpose

   Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

   Depositor

   Diego Orzaez

   Insert

   Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos, 35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

   Plasmid

   #160640

   Purpose

   Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

   Depositor

   Diego Orzaez

   Insert

   Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos, 35S

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. eSpCas9(1.1)_No_FLAG_AAVS1_T2

   Plasmid

   #79888

   Purpose

   Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-Cas9-GFP

   Plasmid

   #86145

   Purpose

   Lentiviral vector expressing Cas9-GFP

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   Cas9-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. DHFR-SpCas9-DHFR

    Plasmid

    #85447

    Purpose

    Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-SpCas9-DHFR
    

    Use

    AAV

    Tags

    Destabilized domain (C-terminus version) of E.col…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.3-SlugCas9-BlastR

    Plasmid

    #220965

    Purpose

    Expresses human codon-optimized SlugCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SlugCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Puro-Cas9 donor

    Plasmid

    #58409

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. MiniCoopR U6:gRNA, mitfa:Cas9

    Plasmid

    #118840

    Purpose

    Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6.3-enCjCas9-BlastR

    Plasmid

    #220978

    Purpose

    Expresses human codon-optimized enCjCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    enCjCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZS157 CRISPEY RT/Cas9

    Plasmid

    #114454

    Purpose

    Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Insert

    SpCas9, Ec86-RT
    

    Use

    CRISPR

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    S. cerevisiae codon optimized

    Promoter

    GAL1-GAL10

    Available Since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Cas9-NRTH

    Plasmid

    #136925

    Purpose

    Expresses Cas9-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9(1.1)-Puro

    Plasmid

    #117686

    Purpose

    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

    Depositor

    Luc Leyns

    Article

    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    PuroR (R166H)

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    U6

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Cas9-NRCH

    Plasmid

    #136926

    Purpose

    Expresses Cas9-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJ609-dCas9-KR-puro

    Plasmid

    #158478

    Purpose

    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

    Depositor

    Brendan Price

    Article

    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

    Insert

    dCas9-KR
    

    Use

    CRISPR

    Tags

    KillerRed

    Expression

    Mammalian

    Mutation

    Cas9m4 (D10A+D839A+H840A+N863A)

    Promoter

    CMV

    Available Since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.3-SaCas9-BlastR

    Plasmid

    #220966

    Purpose

    Expresses human codon-optimized SaCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge