We narrowed to 3,198 results for: ER
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pBXNPHM3
Plasmid#110099PurposeE.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage siteDepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGW_PSD95.FingR-YFP
Plasmid#46299DepositorInsertPSD95.FingR (DLG4 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP1a
Plasmid#205304PurposeExpression of WHaloCaMP1a in E. coliDepositorInsertWHaloCaMP1a
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-mSTING (N153S)
Plasmid#214158PurposeTransient expression of gain-of-function STING mutantDepositorInsertSTING (N153S) (Sting1 Mouse)
UseTagsHAExpressionMammalianMutationN153SPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
meffBlue chromoprotein
Plasmid#117845PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, meffBlue
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC-mCh-P2A-ST-EGFP-CAAX
Plasmid#207636PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tagDepositorInsertphotocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
UseTagsExpressionMammalianMutationAmber stop codon at SpyCatcher’s critical lysinePromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG-DO-TC66T-mCherry
Plasmid#175178PurposeCre-off TVA-mCherry fusion protein with 66T mutationDepositorInsertTVA
UseAAVTagsmCherryExpressionMutationE66TPromoterCAGAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
mDlx-SynTagMA_post-2A-mCerulean
Plasmid#130899PurposeSynaptic Tag for Mapping Activity in GABAergic neuronsDepositorInsertSynTagMA
UseAAVTagsmCeruleanExpressionMammalianMutationPromotermDlxAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-NBK-1
Plasmid#207639PurposeA plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.DepositorInsertPyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
UseTagsExpressionBacterialMutationThe evolved synthetase NBK-1 contains mutations Y…PromoteraraBADAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC208-SYNJ2BP-V5
Plasmid#92149PurposeExpresses V5-tagged human SYNJ2BP in mammalian cells.DepositorInsertSYNJ2BP-V5 (SYNJ2BP Human)
UseLentiviralTagsV5ExpressionMammalianMutation*(see below)PromoterCMVAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaMPARI V398D
Plasmid#60786PurposeExpresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integratorDepositorInsertCaMPARI V398D
UseTagsNuclear Export SignalExpressionMammalianMutationV398DPromoterCMVAvailable sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh
Plasmid#207634PurposeA plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.DepositorInsertphotocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
UseTagsExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMV361_MjTyrRS
Plasmid#193250PurposeExpresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)DepositorInsertMjTyrRS
UseTagsExpressionBacterialMutationPromoterhsp60Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bHs
Plasmid#134259PurposeFor L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tagDepositorInsertHyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
UseTags6x HisExpressionBacterialMutationcodon optimized for Salmonella typhimuriumPromoterAraCAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pYPQ233 (dLbCpf1-SRDX)
Plasmid#86211PurposeDeactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPRTags5' and 3' NLS and SRDX repressorExpressionPlantMutationPromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
amilGFP chromoprotein
Plasmid#117842PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilGFP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES-ManII-mScarlet-i
Plasmid#117274PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-iDepositorInsertsUseTagsmScarlet-iExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMV and IRESAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6m.WPRE.SV40
Plasmid#100841PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6m
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T …PromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only
