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Plasmid#114365PurposeProtein expression plasmid for 3xNLS SpCas9 in pET-21a backboneDepositorInsert3xNLS_SpCas9
UseCRISPRTagsNLSExpressionBacterialPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RNA motif plasmid cloning backbone
Plasmid#107253PurposeRNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kbDepositorInsertRNA motif plasmid cloning backbone
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-AtU6-1
Plasmid#66202Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP Cep170 (Nigg PID200)
Plasmid#41150DepositorAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TOP-GFP
Plasmid#35489DepositorPublicationInsert7xTOP-GFP
UseLentiviralExpressionMammalianPromoter7xTCF/LEFAvailable sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHCMV-MokolaG
Plasmid#15811DepositorInsertMokola virus glycoprotein (G) gene
ExpressionMammalianAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBabe-GFP-IKBalpha-wt
Plasmid#15263DepositorPublicationAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cdk6-HA
Plasmid#1868DepositorAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP-Rab7A
Plasmid#28047DepositorAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP
Plasmid#111585PurposeAn AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyriRFP670V5-P2A-post-eGRASP
UseAAVPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICE-RNaseHI-WT-NLS-mCherry
Plasmid#60365PurposePlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI
TagsNLS from SV40 T antigen and mCherryExpressionMammalianPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas serviceAAV2, AAV5, AAV8, and AAV RetrogradeInsertEGFP
UseAAVPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNW55
Plasmid#22988PurposeExpression of miRNA precursor for the osa-MIR528 locus in rice.DepositorPublicationInsertosa-MIR528
UseRNAi; Plant gene inactivationExpressionPlantAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCALNL-DsRed
Plasmid#13769PurposeCre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.DepositorInsertDsRed2
UseCre/Lox; Flp/frtExpressionMammalianPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro
Plasmid#141392PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf9b
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro
Plasmid#141369PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
CD11b promoter in pGEM3zf(-)
Plasmid#26168DepositorPublicationInsertCD11b promoter (ITGAM Human)
ExpressionBacterialAvailable sinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CKIIa-stGtACR2-FusionRed (AAV Retrograde)
Viral prep#105669-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsFusionRedAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro
Plasmid#141387PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf6 (ORF6 SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by