We narrowed to 8,654 results for: pcDNA3

1. pcDNA3.1.Gαi1-LgB91

   Plasmid

   #134342

   Purpose

   Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαi1-LgB91 (GNAI1 Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-DN-hCUL4B-FLAG

   Plasmid

   #15822

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin4B (CUL4B Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N594

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3 Flag MKK7B2Jnk2a2

   Plasmid

   #19727

   Depositor

   Roger Davis

   Article

   Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

   Insert

   pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Human, Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-DN-hCUL5-FLAG

   Plasmid

   #15823

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin5 (CUL5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N441

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PA-B/BRISBANE/60/2008

   Plasmid

   #187920

   Purpose

   Expresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase acidic protein
   

   Tags

   UNTAGGED

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-300(HAT-)

   Plasmid

   #23254

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Expression

   Mammalian

   Mutation

   H1415A E1423A Y1424A L1428S Y1430A H1434A

   Available Since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-ERK-SPARK

   Plasmid

   #106921

   Purpose

   Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   ERK-SPARK
   

   Expression

   Mammalian

   Available Since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Bsd-mEGFP

   Plasmid

   #214369

   Purpose

   Expression of the monomeric Green Fluorescent Protein

   Depositor

   Bertrand Collet

   Insert

   mEGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-erExRai-AKAR2

   Plasmid

   #161756

   Purpose

   ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   CYP450-ExRai-AKAR2
   

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Gαq*-BERKY3

    Plasmid

    #158217

    Purpose

    BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3-TRIF-CFP

    Plasmid

    #13644

    Depositor

    Doug Golenbock

    Insert

    TRIF (TICAM1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/HA-FLAG-RBP2

    Plasmid

    #14800

    Depositor

    William Kaelin

    Article

    Klose et al Cell. 2007 Mar 9. 128(5):889-900.

    Insert

    Retinoblastoma Binding Protein 2 (KDM5A Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. LRP4-Flag/pCDNA3.1

    Plasmid

    #197291

    Purpose

    Plasmid expressing an antigen targeting LRP4 (extracellular)

    Depositor

    Steve Burden

    Insert

    LRP4 (Lrp4 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Lyn-dInPAkt

    Plasmid

    #181834

    Purpose

    Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dInPAkt
    

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-FLAG-RGS14

    Plasmid

    #236567

    Purpose

    Expresses Flag-RGS14 in mammalian cells

    Depositor

    John Hepler

    Article

    Friedman et al J Biol Chem. 2022 May;298(5):101836. doi

    Insert

    Homo sapiens regulator of G-protein signaling 14 (RGS14 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Ash2L full

    Plasmid

    #15548

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Expression

    Mammalian

    Mutation

    R622H (Please see depositor comments below)

    Available Since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. RGS-6xHis-pcDNA3.1-

    Plasmid

    #52534

    Purpose

    enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Type

    Empty backbone

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Akt-STOPS

    Plasmid

    #175006

    Purpose

    Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.3 Gag-imCherry

    Plasmid

    #208882

    Purpose

    codes for Gag from HIV-1 with an internal mCherry fluorescent protein

    Depositor

    Raphael Gaudin

    Article

    Diana Brychka et al bioRxiv

    Insert

    HIV-1 Gag
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-FLAG-RIPK1

    Plasmid

    #78842

    Purpose

    To overexpress RIPK1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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