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Showing: 3851 - 3900 of 5144 results

  1. pOCC93

    Plasmid
    #118911
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118911-plasmid-map-sequence-id-232347

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  2. pET28a-HsSENP5

    Plasmid
    #71468
    Purpose
    Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 5 (SENP5 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pSCM167.2-Venus-VF-hADAM10(aa455-550)

    Plasmid
    #189556
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 455-550
    Promoter
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189556-plasmid-map-sequence-id-384141

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  4. pSCM167-Venus-VF-hADAM10(aa220-459)

    Plasmid
    #189555
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 220-459
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189555-plasmid-map-sequence-id-374397

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  5. pSCM167.2-Venus-VF-hADAM10(aa551-654)

    Plasmid
    #189557
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only cysteine rich domain--amino acids 551-654
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa551-654
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189557-plasmid-map-sequence-id-374394

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  6. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid
    #189562
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa 1-213
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189562-plasmid-map-sequence-id-374127

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  7. pSCM167-Venus-VF-hADAM10(aa455-654)

    Plasmid
    #189561
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 455-654
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    189561-plasmid-map-sequence-id-374541

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  9. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid
    #167169
    Purpose
    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 (Apobec1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Leucine 173 to Alanine; changed Glycine 2…
    Promoter
    CMV promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    167169-plasmid-map-sequence-id-335363

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  10. pOCC121

    Plasmid
    #118893
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and MBP, cleavab…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118893-plasmid-map-sequence-id-232270

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  14. pOCC70

    Plasmid
    #118878
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    mGFP, cleavable with TEV protease; HIS6, cleavabl…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118878-plasmid-map-sequence-id-232355

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  16. pLyGo-Ec-6

    Plasmid
    #163132
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163132-plasmid-map-sequence-id-338662

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  21. pOCC92

    Plasmid
    #118910
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    118910-plasmid-map-sequence-id-234552

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  22. pOCC6

    Plasmid
    #118864
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, EGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118864-plasmid-map-sequence-id-232291

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  23. pOCC81

    Plasmid
    #118900
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GST
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease; GST tag
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118900-plasmid-map-sequence-id-232307

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  24. pOCC204

    Plasmid
    #118894
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and mKate2, HIS6,…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118894-plasmid-map-sequence-id-232313

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  29. L3MBTL3 (3UT1)

    Plasmid
    #36901
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3 (L3MBTL3 Human)
    Use
    Tags
    His tag and Streptavidin-Binding Peptide (SBP)-Ta…
    Expression
    Bacterial
    Mutation
    contains amino acid residues 228-551 only
    Promoter
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pOCC215

    Plasmid
    #118879
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    Dendra2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118879-plasmid-map-sequence-id-232264

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  32. pOCC207

    Plasmid
    #118869
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mCherry
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118869-plasmid-map-sequence-id-232318

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  34. pLaNGo11-3

    Plasmid
    #126054
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126054-plasmid-map-sequence-id-254921

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  36. pOCC230

    Plasmid
    #118871
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and mCitrine, cl…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118871-plasmid-map-sequence-id-232332

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  37. pOCC69

    Plasmid
    #118874
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and mGFP, cleava…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118874-plasmid-map-sequence-id-232330

    Enlarge

  38. pOCC110

    Plasmid
    #118905
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and LPETGg
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118905-plasmid-map-sequence-id-232327

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  39. pOCC219

    Plasmid
    #118908
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
    Use
    Tags
    YBBR tag, HIS10, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118908-plasmid-map-sequence-id-232256

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  42. pEG610

    Plasmid
    #40195
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa 1-355; exon 2 recoded to be RNAi-resi…
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only

  43. NLuc-His10

    Plasmid
    #234567
    Purpose
    Bacterially expressed NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Nano luciferase
    Use
    Tags
    T7 gene 10 leader peptide and Thrombin site-10xHi…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234567-plasmid-map-sequence-id-466796

    Enlarge

  44. pLD-puro-Cc-IFIT5_K206R-VA

    Plasmid
    #191224
    Purpose
    Lentiviral expression of human IFIT5_K206R in mammalian cells
    Depositor
    Mohan Babu
    Insert
    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    Use
    Lentiviral
    Tags
    3xFlag-TEVx2-6xHis-Strep x2 tag and VA tag
    Expression
    Mammalian
    Mutation
    changed Lysine 206 to Arginine (K206R)
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191224-plasmid-map-sequence-id-438791

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Showing: 3851 - 3900 of 5144 results