We narrowed to 4,933 results for: gne

1. pGK:HygroR-CMV:mKate2-B_UTR

   Plasmid

   #68480

   Purpose

   Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mKate2-B_UTR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "B" added to 3&#x…

   Promoter

   CMV and pGK

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. C3 luc (T1 del)

   Plasmid

   #11358

   Depositor

   Donald McDonnell

   Article

   Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

   Insert

   C3 promoter (C3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN1-CMV-sDarken

   Plasmid

   #184799

   Purpose

   Serotonin Sensor sDarken under the control of a CMV Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   sDarken
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTpPuc3

   Plasmid

   #62864

   Purpose

   Episome vector for Thalassiosira pseudonana (pUC-based)

   Depositor

   Hamilton Smith

   Article

   Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Episomal vector for thalassios…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCit-APPAPAPPAP-gs

   Plasmid

   #228091

   Purpose

   Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells

   Depositor

   Roman Jerala

   Article

   Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

   Insert

   mCit, APPAPAPPAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1neo-NLucYAP15

   Plasmid

   #107610

   Purpose

   measure LATS activity by Luciferase Assay

   Depositor

   Xiaolong Yang

   Article

   Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

   Insert

   NLucYAP15
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mucin MUC1-5TR-pET15b

   Plasmid

   #174694

   Purpose

   Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

   Depositor

   Jean Gariepy

   Article

   Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

   Insert

   MUC1-TR
   

   Use

   Tags

   hexahistidine (HIS) tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS-u(M)Cas9

   Plasmid

   #127384

   Purpose

   Expresses Cas9 at medium level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(M)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1neo-14-3-3-CLuc

   Plasmid

   #107611

   Purpose

   measure LATS activity by Luciferase Assay

   Depositor

   Xiaolong Yang

   Article

   Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

   Insert

   pCDNA3.1hygro-BamHI-CLuc1433-NotI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMito-miRFP718nano

    Plasmid

    #195754

    Purpose

    Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    Human cytochrome C oxidase subunit VIII-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDS275

    Plasmid

    #34972

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A, I532A

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH2B-miRFP718nano

    Plasmid

    #195750

    Purpose

    Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    H2B-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-Promoter-4xBS_BHLHE40

    Plasmid

    #153064

    Purpose

    Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    4X consensus BHLHE40 binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-NavMs

    Plasmid

    #100004

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP

    Depositor

    Paul DeCaen

    Article

    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10

    Insert

    His-NavMs, Voltage gated sodium channel
    

    Use

    Tags

    Hexa-His tag followed by a thrombin cleavage site

    Expression

    Mammalian

    Mutation

    C-terminus from a codon-optimized synthetic gene,…

    Promoter

    CMV

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS306-pHIS3-2xGFP-tURA3

    Plasmid

    #116936

    Purpose

    Yeast integrative vector for the expression of 2xGFP

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    2xGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40

    Plasmid

    #100831

    Purpose

    AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Loren Looger, Eric Schreiter

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Insert

    CaMPARI
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLO 64.1

    Plasmid

    #123120

    Purpose

    E. coli expression vector for active deltaproteobacteria CasX

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDUP1

    Plasmid

    #223516

    Purpose

    inactive cogfp under control of Ptet, cogfp under control of Ptac

    Depositor

    Yolanda Schaerli

    Article

    Ljiljana et al eLife13:RP97216

    Inserts

    CoGFP
    CoGFP inactivated
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    anti-dimerization mutations S129G, 22 C154S, D156…

    Promoter

    Ptac, Ptac and Ptet, and Ptet

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. RC(WW)-LgBit

    Plasmid

    #208931

    Purpose

    Recognition domain for in vitro MAPK-activity sensor

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    WW domain of PIN1 (PIN1 Human)
    

    Use

    Tags

    HISx6, LgBit, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCit-APPAP-gs

    Plasmid

    #228090

    Purpose

    Mammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCit, APPAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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