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We narrowed to 299 results for: IPTG

Showing: 281 - 299 of 299 results

  1. pRARE-MBP-DEST

    Plasmid
    #84650
    Purpose
    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.
    Depositor
    Neil Olszewski
    Article
    Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:
    Type
    Empty backbone
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    84650-plasmid-map-sequence-id-162112

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  7. pETM11-6xHis-TEV-hORF1p

    Plasmid
    #202589
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Kathleen Burns, Liam Holt
    Article
    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    202589-plasmid-map-sequence-id-413699

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  8. TriEx-4 DHX36

    Plasmid
    #68368
    Purpose
    Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.
    Depositor
    James Vaughn
    Article
    Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2
    Insert
    DHX36 (DHX36 Human)
    Use
    Tags
    Histidine
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV ie enhancer/promoter
    Available since
    Sept. 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Ryan Mehl, Richard Cooley
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  11. pGEX-2T-TRX-C751-E3616S

    Plasmid
    #182844
    Purpose
    mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2014 Mar;141(5):1129-39. do
    Insert
    TRX (w Fly)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182844-plasmid-map-sequence-id-360053

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Showing: 281 - 299 of 299 results