We narrowed to 8,788 results for: Ott

1. pcDNA3-3myc-6His-EZH2 21D

   Plasmid

   #42664

   Depositor

   Mien-Chie Hung

   Article

   Cha et al Science. 2005 Oct 14;310(5746):306-10.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   3Myc and 6His

   Expression

   Mammalian

   Mutation

   S21D

   Promoter

   Available since

   July 17, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-3myc-6His-EZH2 21A

   Plasmid

   #42663

   Depositor

   Mien-Chie Hung

   Article

   Cha et al Science. 2005 Oct 14;310(5746):306-10.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   3Myc and 6His

   Expression

   Mammalian

   Mutation

   S21A

   Promoter

   Available since

   July 17, 2013

   Availability

   Academic Institutions and Nonprofits only

3. PDGFR-TMD (MTK 3B) (pZYW025)

   Plasmid

   #194218

   Purpose

   Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PDGFR TMD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. T2A-mCherry (MTK 4a) (pAN3844)

   Plasmid

   #194223

   Purpose

   Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   T2A-mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

   Plasmid

   #194229

   Purpose

   Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PGK-LCB1-SynNotch-T2A-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mEmerald-MyosinIIA-N-14

   Plasmid

   #54191

   Purpose

   Localization: Cytoskeleton, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

   Insert

   MyosinIIA
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. CoV2-N-R203K

   Plasmid

   #177951

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203K) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R203K

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CoV2-N-G204R

   Plasmid

   #177953

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (G204R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G204R

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEmerald-MyosinIIA-C-18

   Plasmid

   #54190

   Purpose

   Localization: Cytoskeleton, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

   Insert

   MyosinIIA
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pMZ827

    Plasmid

    #155321

    Purpose

    Constitutive expression of E-PIF6(1-100) in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-E-PIF6(1-100)-NLS-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pROF394

    Plasmid

    #155333

    Purpose

    Expression of PULSE-controlled LFY-VP16-2A-RLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
    

    Use

    Synthetic Biology; Eukaryotic expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CoV2-N-D3L

    Plasmid

    #177945

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D3L

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CoV2-N-A119S

    Plasmid

    #177948

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (A119S) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A119S

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mApple-MyosinIIA-N-18

    Plasmid

    #54930

    Purpose

    Localization: Cytoskeleton, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

    Insert

    MyosinIIA
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CoV2-N-M234I

    Plasmid

    #177955

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (M234I) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M234I

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CoV2-N-D377Y

    Plasmid

    #177957

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D377Y) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D377Y

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CoV2-N-P13L

    Plasmid

    #177946

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P13L) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P13L

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CoV2-N-P80R

    Plasmid

    #177947

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P80R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CoV2-N-S235F

    Plasmid

    #177956

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (S235F) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S235F

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEos2-MyosinIIA-C-18

    Plasmid

    #57403

    Purpose

    Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

    Insert

    MyosinIIA
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

21. PGK Promoter (MTK 2) (pAN2822)

    Plasmid

    #194224

    Purpose

    Mammalian Toolkit part 2 encoding the PGK promoter

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EF1a Promoter (MTK 2) (pAN2824)

    Plasmid

    #194225

    Purpose

    Mammalian Toolkit part 2 containing the EF1a promoter

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMZ828

    Plasmid

    #155322

    Purpose

    Constitutive expression of PhyB(1-650)-VP16 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pROF021

    Plasmid

    #155326

    Purpose

    Expression of PULSE-controlled FLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-FLuc-TSV40
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pROF366

    Plasmid

    #155332

    Purpose

    Expression of FLuc under the control of AtAP1 promoter

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PAtAP1-FLuc-TSV40
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CoV2-Spike-D614G

    Plasmid

    #177960

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CoV2-M-IRES-E

    Plasmid

    #177938

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-FLAG-CRBN-pGK-HYG

    Plasmid

    #107380

    Purpose

    Mammalian expression of FLAG-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. plenti-UbcP-HA-CRBN-pGK-HYG

    Plasmid

    #107378

    Purpose

    Lentiviral expression of HA-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UbcP

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Luc-T20

    Plasmid

    #177941

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. plenti-CAG-IRF4-FLAG-IRES-GFP

    Plasmid

    #107389

    Purpose

    Lentiviral expression of IRF4-FLAG

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid

    #177939

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G, N501Y

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Luc-PS9

    Plasmid

    #177942

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC19_CoV-2_linker

    Plasmid

    #211731

    Purpose

    Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

    Depositor

    Thomas Klimkait

    Article

    Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

    Insert

    Linker fragment SARS-CoV-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CoV2-N-WT-Hu1

    Plasmid

    #177937

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pROF148

    Plasmid

    #155330

    Purpose

    Binary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette. 

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_Tnos-nptII-Pnos_PAtUbi10-SRDX-NLS-EL222-Tnos_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

    Plasmid

    #194231

    Purpose

    Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a-LCB1-PDGFR TMD-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. plenti-CAG-IKZF3-FLAG-IRES-GFP

    Plasmid

    #107388

    Purpose

    Lentiviral expression of IKZF3-FLAG

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. plenti-UBC- IKZF4-3xHA-pGK-PUR

    Plasmid

    #107384

    Purpose

    Lentiviral expression of IKZF4-3xHA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF4 (IKZF4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid

    #60866

    Purpose

    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    CohIII
    CBM
    

    Use

    Tags

    6xHIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Cysteine 63 to Serine in CBM module

    Promoter

    T7

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid

    #60865

    Purpose

    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    Xyl
    Xmod-DocIII
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Threonine 120 to Cysteine in Xylanase domain

    Promoter

    T7

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3-IKZF2-V2-V5

    Plasmid

    #107391

    Purpose

    Mammalian expression of IKZF2-V2-V5

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF2-V2 (IKZF2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3-IKZF1-V2-V5

    Plasmid

    #107390

    Purpose

    Mammalian expression of IKZF1-V2-V5

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1-V2 (IKZF1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. plenti-px330-CRBN-T1-pGK-Pur

    Plasmid

    #107382

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. CoV2-N-P.1

    Plasmid

    #177959

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P80R,R203K,G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pIRIGF-IKZF3

    Plasmid

    #69046

    Purpose

    expression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    IRES

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

    Plasmid

    #194222

    Purpose

    Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    SynNotch-GAL4 DBD-VP64 AD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. plenti-UbcP-FLAG-CRBN-pGK-HYG

    Plasmid

    #107374

    Purpose

    Lentiviral expression of FLAG-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    UbcP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. plenti-UBC- IRF4-3xHA-pGK-PUR

    Plasmid

    #107386

    Purpose

    Lentiviral expression of IRF4-3xHA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge