We narrowed to 29,658 results for: REP

1. pREP-NFAT2

   Plasmid

   #11788

   Depositor

   Anjana Rao

   Insert

   NFAT2 (NFATC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cre Reporter

   Plasmid

   #62732

   Purpose

   Mammalian Cre recombinase reporter

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   LoxP-DsRED-STOP-LoxP-eGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   LoxP sites

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-Alpha

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-StrepII

   Plasmid

   #208617

   Purpose

   Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Tags

   Strep II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 2C::tdTomato Reporter

   Plasmid

   #40281

   Depositor

   Samuel Pfaff

   Article

   Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

   Insert

   2C Promoter (2-cell stage promoter)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. PM PKA Reporter

   Plasmid

   #104551

   Purpose

   Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Lyn-mTurquoise2-VASP 148 - 164
   

   Use

   Tags

   mTurquoise 2, HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. miReporter-PGK

   Plasmid

   #82477

   Purpose

   microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   H2B-mCherry and H2B-Citrine. HygroR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29b-H6_Streptavidin_sfGFP

   Plasmid

   #124296

   Purpose

   His6-tagged Streptavidin GFP fusion protein

   Depositor

   Mark Arbing

   Insert

   His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
   

   Use

   Tags

   His6 and T7 tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. miReporter-CAG

   Plasmid

   #82478

   Purpose

   microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   H2B-mCherry and H2B-Citrine. HygroR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTD-NTwinStrep_Km

   Plasmid

   #45938

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. reporter-gT1

    Plasmid

    #47320

    Purpose

    TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    minCMV-dTomato_pA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_StrepII_TEV_LIC_TniQ(PmcCAST)

    Plasmid

    #224927

    Purpose

    TniQ expression plasmid for the co-purification of TnsC-TniQ complex

    Depositor

    Leifu Chang

    Article

    Shukun et al Academic Press, Volume 712, 2025, Pages

    Insert

    TniQ
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTD-CTwinStrep

    Plasmid

    #45939

    Purpose

    use to create C-terminal Twin-Strep-tag fusion proteins

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTD-NStrepHis

    Plasmid

    #45936

    Purpose

    use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB1677 REPAIR.t3

    Plasmid

    #176320

    Purpose

    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q, only the deaminase domain (aa 276-702 are …

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. lucMAPT-GenRep

    Plasmid

    #214674

    Purpose

    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAB1923 REPAIR.t1-S

    Plasmid

    #176321

    Purpose

    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q/E620G/Q696L, only the deaminase domain (aa …

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-3'TwStrep

    Plasmid

    #208053

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-5'TwStrep

    Plasmid

    #208620

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P1-N-Strep2

    Plasmid

    #119758

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST and Strep-tag II fusion tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. RGB-S reporter

    Plasmid

    #207841

    Purpose

    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

    Depositor

    Christof Niemeyer

    Article

    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

    Inserts

    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PosmY, PsulA, and grpE

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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