We narrowed to 647 results for: SPY

1. pDEST14-SpyCatcher EQ

   Plasmid

   #35045

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyCatcher EQ
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Deletion of C-terminal till I113, and point mutat…

   Promoter

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pDEST14-SpyCatcher002 EQ

   Plasmid

   #102830

   Purpose

   Bacterial expression of unreactive version of SpyCatcher002

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002 EQ
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   E77Q mutation prevents isopeptide bond formation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SpyCas9-AIDx

   Plasmid

   #135343

   Purpose

   Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ166-iSpyMac

   Plasmid

   #149553

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   iSpyMacCas9
   

   Use

   CRISPR; Gateway compatible zispymaccas9 entry clo…

   Tags

   3X FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   R221K, N394K, Protospacer interacting domain form…

   Promoter

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpyTag-GlpG

   Plasmid

   #79054

   Purpose

   Expresses SpyTagged GlpG protein

   Depositor

   James Bowie

   Article

   Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

   Insert

   SpyTag-GlpG (glpG )
   

   Use

   Tags

   6xHis and SpyTag

   Expression

   Bacterial

   Mutation

   GlpG protein employed here corresponds to residue…

   Promoter

   Trc

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAW-SpyCsn2

   Plasmid

   #113251

   Purpose

   E. coli expression vector for S. pyogenes Csn2-MBP-His

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   S. pyogenes Csn2
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCG-B-SpyTag-C

   Plasmid

   #87373

   Purpose

   An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   SpyTag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAW-SpyCas1

   Plasmid

   #113248

   Purpose

   E. coli expression vector for His-MBP-S. pyogenes Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   S. pyogenes Cas1
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW-SpyCas2

   Plasmid

   #113249

   Purpose

   E. coli expression vector for His-MBP-S. pyogenes Cas2

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   S. pyogenes Cas2
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCG-B-mSpyCatcher-C

    Plasmid

    #87374

    Purpose

    An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    mSpyCatcher
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPaint-SpyTag

    Plasmid

    #67176

    Purpose

    CRISPaint universal donor for SpyTag fusion tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    SpyTag
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLIC.B4.SPy0289

    Plasmid

    #11534

    Depositor

    Sung-Hou Kim

    Article

    Liu et al Proteins. 2005 Jun 1. 59(4):875-81.

    Insert

    IscU
    

    Use

    Tags

    HisTag-MBP, TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pAX1_Z11 gpN:SpyTag

    Plasmid

    #225196

    Purpose

    Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTn7xTS_SpyCatcher003:mNG

    Plasmid

    #225190

    Purpose

    Used for inserting SpyCatcher003 fused to mNeonGreen at the bacterial Tn7 site

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    SpyCatcher003:mNeonGreen
    

    Use

    Tags

    SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SpyCatcher-P-Domain

    Plasmid

    #201193

    Purpose

    Expresses furin P-domain genetically fused to SpyCatcher001 in bacteria

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

    Insert

    SpyCatcher-P-Domain
    

    Use

    Tags

    HisTag and SpyCatcher001

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-AP-SpyCatcher

    Plasmid

    #223636

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His10, AP fragment of split APEX2 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEC2796 (pSpy0C4)

    Plasmid

    #220280

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpyA-GFP

    Plasmid

    #220189

    Purpose

    sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pEM7 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pEM7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpyB-GFP

    Plasmid

    #220190

    Purpose

    sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG35

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpyD-GFP

    Plasmid

    #220192

    Purpose

    sgRNA expression vector - pBG28 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG28 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG28

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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