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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,383 results for: mpl

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Showing: 2371 - 2400 of 11383 results

  2. pATP416-­pluxO5-­pphlO6-­crtYBI

    Plasmid
    #165977
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165977-plasmid-map-sequence-id-328856

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  3. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid
    #183081
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Mutation
    -146C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183081-plasmid-map-sequence-id-363415

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  4. pBFC0625

    Plasmid
    #186691
    Purpose
    VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_lacZ_α_1 Spacer crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186691-plasmid-map-sequence-id-371386

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  7. pGL4.75-KRAS LCS6m

    Plasmid
    #44571
    Depositor
    Frank Slack
    Insert
    KRAS 3'UTR (KRAS Human)
    Expression
    Mammalian
    Mutation
    The T allele at LCS6 (let-7 complementary site 6)…
    Promoter
    CMV
    Available Since
    April 12, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pD649-HAsp-CD33-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156694
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD33 (CD33 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156694-plasmid-map-sequence-id-393026

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  9. ppk301-T2A-QF2 HDR plasmid

    Plasmid
    #130667
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene
    Depositor
    Leslie Vosshall
    Article
    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1
    Insert
    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    Use
    Donor template
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    130667-plasmid-map-sequence-id-257080

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  13. pD649-HAsp-PDCD1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156543
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    PDCD1 (PDCD1 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156543-plasmid-map-sequence-id-368511

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  14. 6xHis-MBP-huErCas12a

    Plasmid
    #174685
    Purpose
    Expresses 6xHis-MBP tagged ErCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huErCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis
    Expression
    Bacterial
    Mutation
    Q926R mutation is observed as indicated from the …
    Promoter
    T7
    Available Since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    174685-plasmid-map-sequence-id-346198

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  15. AICSDP-72:TFAM-mEGFP

    Plasmid
    #159745
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TFAM
    Depositor
    Allen Institute for Cell Science
    Insert
    TFAM Homology Arms with linker-mEGFP (TFAM Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Oct. 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159745-plasmid-map-sequence-id-314375

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  17. pCW-eGFP-FHA-SHLD2-m1

    Plasmid
    #114122
    Purpose
    Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    SHLD2 (m1 mutant) (SHLD2 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    RNF8 FHA domain (aa 2-160) and eGFP
    Expression
    Mammalian
    Mutation
    W489A, F494A, W495A mutations introduced
    Promoter
    TRE promoter, Tet ON
    Available Since
    Aug. 28, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114122-plasmid-map-sequence-id-220204

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  20. YFP(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #54470
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP(1-158)/beta-5 (GNB5 Aequorea victoria, Human)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54470-plasmid-map-sequence-id-114865

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  25. pAAH1

    Plasmid
    #41825
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7, TEF
    Available Since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41825-plasmid-map-sequence-id-56469

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  26. pAAH6

    Plasmid
    #41830
    Purpose
    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7,TEF
    Available Since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41830-plasmid-map-sequence-id-69212

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Showing: 2371 - 2400 of 11383 results