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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,860 results for: 158

Showing: 1581 - 1600 of 9860 results

  1. pCfB6679

    Plasmid
    #106158
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106158-plasmid-map-sequence-id-206236

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  5. Nabi2.242

    Plasmid
    #73754
    Purpose
    Nabi2.242 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Article
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Insert
    Nabi2.242
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73754-plasmid-map-sequence-id-136686

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  6. pAN1935

    Plasmid
    #127203
    Purpose
    Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    127203-plasmid-map-sequence-id-250001

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  8. pAN1232

    Plasmid
    #127164
    Purpose
    Vector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)
    Depositor
    Hana El-Samad
    Article
    Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    127164-plasmid-map-sequence-id-250325

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  16. EW394 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-(GGS)x8-FKBP (FLP-IN)

    Plasmid
    #236158
    Purpose
    Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a GS linker to FKBP1A, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ZNF35(5-8)-ZNF250(1-4)-FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236158-plasmid-map-sequence-id-468402

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  20. pHBJT007

    Plasmid
    #225158
    Purpose
    Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    225158-plasmid-map-sequence-id-446507

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Showing: 1581 - 1600 of 9860 results