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pCX459

Plasmid

#229214

Purpose

CRISPR array plasmid for MAP1B (Spacers 1-24)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 1-24)

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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multi-shRNA vector

Plasmid

#12391

Depositor

Zuoshang Xu

Article

Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Insert

mSOD2 shRNA (Sod2 Mouse)

Use

RNAi

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pTK222

Plasmid

#13532

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Tags

See map

Expression

Mammalian

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pEMG-AIS

Plasmid

#225133

Purpose

For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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BCJJ163

Plasmid

#117512

Purpose

AtRPS5a promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pRPS5a:AATG:BsaI

Expression

Plant

Promoter

AtRPS5a

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LBJJ491

Plasmid

#117513

Purpose

AtYAO promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pYAO:AATG:BsaI

Expression

Plant

Promoter

AtYAO

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCX460

Plasmid

#229215

Purpose

CRISPR array plasmid for MAP1B (Spacers 25-48)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 25-48)

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTHSSe_50

Plasmid

#109248

Purpose

incW BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Backbone(NG)

Plasmid

#27963

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NG)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMCSG17-NleL

Plasmid

#66716

Purpose

Expresses NleL (170-782) in E. coli

Depositor

David Komander

Article

Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

Insert

NleL (NEWENTRY )

Tags

GST-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
pSFV3-lacZ

Plasmid

#92074

Purpose

This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

lacZ

Use

Lacz expression plasmid, for run-off transcriptio…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pKmK.P2

Plasmid

#125035

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PDC1 promoter

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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GGE0029

Plasmid

#120730

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Type

Empty backbone

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET-PfuX7

Plasmid

#182364

Purpose

Expresses PfuX7 DNA polymerase

Depositor

Morten Norholm

Article

Norholm et al BMC Biotechnol. 2010 Mar 16;10:21. doi:

Insert

PfuX7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Mutation

V93Q

Promoter

T7

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKmK.T4

Plasmid

#125056

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PDC1 terminator

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.P7

Plasmid

#125040

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

TEF1 promoter

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pKL2315

Plasmid

#187876

Purpose

Expresses Cre recombinase.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

J23107

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJOE_pvdJ

Plasmid

#110142

Purpose

Suicide plasmid with repair template for knockout in P. putida

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Type

Empty backbone

Expression

Bacterial

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR.hU6hH1

Plasmid

#32686

Purpose

dual shRNA expression cassette entry vector

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

RNAi

Expression

Mammalian

Promoter

hU6hH1

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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