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pAW156

Plasmid

#113185

Purpose

E. coli expression vector for untagged E. coli Cas1 K259E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

K259E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW186

Plasmid

#113184

Purpose

E. coli expression vector for untagged E. coli Cas1 R163A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R163A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW151

Plasmid

#113183

Purpose

E. coli expression vector for untagged E. coli Cas1 R146A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R146A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW150

Plasmid

#113182

Purpose

E. coli expression vector for untagged E. coli Cas1 S143A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

S143A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW174

Plasmid

#113181

Purpose

E. coli expression vector for untagged E. coli Cas1 R138A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW189

Plasmid

#113180

Purpose

E. coli expression vector for untagged E. coli Cas1 Q136A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

Q136A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW185

Plasmid

#113179

Purpose

E. coli expression vector for untagged E. coli Cas1 R132A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R132A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW184

Plasmid

#113176

Purpose

E. coli expression vector for untagged E. coli Cas1 R112A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R112A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW152

Plasmid

#113175

Purpose

E. coli expression vector for untagged E. coli Cas1 K12E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

K12E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW158

Plasmid

#113177

Purpose

E. coli expression vector for untagged E. coli Cas1 R112E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R112E

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pROP-PP-FBFP

Plasmid

#122137

Purpose

FMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

FBFP

Expression

Bacterial

Promoter

J23101

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-SA-2A-NEO-CAG-RTTA3

Plasmid

#60431

Purpose

neomycin resistance; constitutive promoter driving reverse tet activator

Depositor

Paul Gadue

Article

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

rtTA3

Use

Zfn donor

Promoter

CAG

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

Plasmid

#177203

Purpose

encoding stblHIF under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

stblHIF

Use

Lentiviral

Expression

Mammalian

Mutation

P402A/P577G/N813A

Promoter

TRE

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCYP03

Plasmid

#212836

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLRD04

Plasmid

#208258

Purpose

Production of ent-kaurene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
blr2150

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1his p66shc (corrected)

Plasmid

#32574

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pCYP10

Plasmid

#212843

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSSa29

Plasmid

#179503

Purpose

Tet-on red-shifted luciferase expression

Depositor

Dominique Sanglard

Article

Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

Insert

Red-shifted luciferase

Promoter

Tet-HOP1 (p98CGH)

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB3236

Plasmid

#124708

Purpose

Expresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS416

Depositor

Michael Jensen

Article

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

yEGFP

Promoter

pTRX2_5xUAS

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLRD11

Plasmid

#208263

Purpose

Production of sclareol in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
abCAS
ssSS

Expression

Bacterial

Mutation

abCAS(D537A)

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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