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pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

Plasmid

#199359

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOEu-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-zeo_3xHA-TurboID-NLS

Plasmid

#200022

Purpose

For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

3xHA-TurboID-NLS

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3Gs

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-SHH

Plasmid

#134350

Purpose

Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

Depositor

Lorenz Studer

Article

Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

Insert

SHH (SHH Human)

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_MYRF

Plasmid

#64843

Purpose

A lentiviral vector for the expression of mouse MYRF under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

MYRF (Myrf Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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Puro-iNgn3

Plasmid

#75337

Purpose

doxycycline inducible Ngn3 over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

neurogenin 3 (Neurog3 Mouse)

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-CDC25A

Plasmid

#216511

Purpose

Expresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CDC25A (CDC25A Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSLIK DN Rac1

Plasmid

#84642

Purpose

Lentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Dominant Negative

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-Relb

Plasmid

#139810

Purpose

doxycycline-inducible expression of mouse Relb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Relb (Relb Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Olig2

Plasmid

#64842

Purpose

A lentiviral vector for the expression of mouse Olig2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSLIK CA Rac1

Plasmid

#84605

Purpose

Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively Active, Q61L

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
XLone-SOX17

Plasmid

#135764

Purpose

expresses human SOX17 upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

SOX17 (SOX17 Human)

Expression

Mammalian

Mutation

none

Promoter

TRE3G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Divergent

Plasmid

#229791

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_Divergent Donor

Plasmid

#229784

Purpose

Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-SOX18

Plasmid

#163822

Purpose

expresses human SOX18 upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

SOX18 (SOX18 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMVtight-RFP-IRES-CD

Plasmid

#126678

Purpose

Doxycycline inducible expression of CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

RFP-IRES-CD (FCY1 Budding Yeast)

Promoter

tight TRE

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-TetO-PM

Plasmid

#197387

Purpose

To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

PDX1-T2A-MAFA

Use

Lentiviral; Teto

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Puro-iNotch1IC

Plasmid

#75338

Purpose

doxycycline inducible Notch1 intracellular domain gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

Notch1 Intracellular domain (Notch1 Mouse)

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

Plasmid

#199360

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOEu-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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