We narrowed to 4,154 results for: pCMV

1. pCMV-PE2-P2A-BSD

   Plasmid

   #174819

   Purpose

   Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PE2-P2A-BSD
   

   Use

   Tags

   P2A-BSD and SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-myc-ERK2-MEK1_fusion

   Plasmid

   #39194

   Depositor

   Melanie Cobb

   Article

   Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

   Insert

   ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-PE2-tagRFP-BleoR

   Plasmid

   #192508

   Purpose

   Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

   Depositor

   Gerald Schwank

   Article

   Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   PE2-P2A-tagRFP-P2A-BleoR
   

   Use

   Tags

   BleoR, SV40 bpNLS, and tagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-dCas12a-ABE8e-V106W

   Plasmid

   #193646

   Purpose

   Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA*8e-V106W, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TadA*8e-V106W

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EYFP-hDF-deltaTM-pCMV-9

   Plasmid

   #72586

   Purpose

   human dysferlin no transmembrane domain tagged with YFP

   Depositor

   Renzhi Han

   Article

   Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

   Insert

   dysferlin (DYSF Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   transmembrane domain deleted

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PCMV-intron myc Rab2WT

   Plasmid

   #46779

   Purpose

   expression of WT Rab2a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB2 (RAB2A Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCMV6-XL4 ASXL1 (1-1304)

   Plasmid

   #74244

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains ASXL1 amino acids 1-1304

   Promoter

   CMV

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCMV5B-TGFbeta receptor II wt (N-term HA)

   Plasmid

   #24801

   Depositor

   Jeff Wrana

   Insert

   TGFbeta receptor II (TGFBR2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pCMV-Tag2B EDD C2768A

   Plasmid

   #37189

   Depositor

   Charles Watts, Darren Saunders

   Article

   Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

   Insert

   EDD C2768A (UBR5 Human)
   

   Use

   Tags

   6x His Tag and FLAG

   Expression

   Mammalian

   Mutation

   Changed Cysteine 2768 to Alanine

   Promoter

   CMV

   Available since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-BE4-PpAPOBEC1 R33A

    Plasmid

    #138345

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1 R33A

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1 R33A
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Change of Arg 33 to Ala.

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-RosaR4 KKR mutations

    Plasmid

    #37199

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to RosaR4 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs R4, and SV40 NLS

    Expression

    Mammalian

    Mutation

    I538K, E490K, and H537R

    Promoter

    CMV

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nxn-1 beta Flag/pCMV

    Plasmid

    #197339

    Purpose

    Plasmid expressing an antigen targeting Neurexin 1beta

    Depositor

    Thomas Südhof

    Insert

    Neurexin 1beta (Nrxn1 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-SARS-CoV-2-Nsp3PLpro

    Plasmid

    #204208

    Purpose

    Mammalian expression of SARS-CoV-2 Nsp3

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
    

    Use

    Tags

    FLAGx1

    Expression

    Mammalian

    Mutation

    PLpro of Nsp3

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-OptoSTIM1(Cry2(D387A))

    Plasmid

    #70160

    Purpose

    Light-insensitive mutant of OptoSTIM1

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1(Cry2(D387A)) (STIM1 Human, Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Neo-Bam E2F4

    Plasmid

    #37966

    Depositor

    Jacqueline Lees

    Article

    Moberg et al Mol Cell Biol. 1996 Apr;16(4):1436-49.

    Insert

    E2F4 (E2F4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Neo-Bam E2F6

    Plasmid

    #37965

    Depositor

    Jacqueline Lees

    Article

    Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

    Insert

    E2F6 (E2F6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163562

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Neo-Bam E2F3b

    Plasmid

    #37975

    Depositor

    Jacqueline Lees

    Insert

    E2F3b (E2F3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-SaPE2-P2A-BSD

    Plasmid

    #174818

    Purpose

    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2-P2A-BSD
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Sport6-CD9-pHuji

    Plasmid

    #130906

    Purpose

    Expression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHuji (CD9 Human, Synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Asn50 and Asn51 in extrace…

    Promoter

    CMV

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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