Addgene

We narrowed to 9,781 results for: otos

Showing: 2431 - 2460 of 9781 results

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LRG2.1-BFP

Plasmid

#120577

Purpose

Lentiviral plasmid to co-express a guide RNA and EBFP.

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET42b-AncBE4max

Plasmid

#165157

Purpose

Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression

Depositor

Taekjip Ha

Article

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

AncBE4max

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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SlugMyc_pcDNA3

Plasmid

#31698

Depositor

Paul Wade

Article

Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

Insert

Slug (SNAI2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pSO2002

Plasmid

#25842

Depositor

Aziz Sancar

Article

Ozgur et al Biochemistry. 2003 Mar 18. 42(10):2926-3

Insert

human Cryptochrome 2 (CRY2 Human)

Use

Tags

flag, his, and myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

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pRS-shTGFbeta1

Plasmid

#14973

Depositor

Joan Massague

Article

Thomas et al Cancer Cell. 2005 Nov . 8(5):369-80.

Insert

TGFbeta1 shRNA (Tgfb1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pAd5-B1-deltaE1-GFP

Plasmid

#179203

Purpose

Block 1 with deletion in E1 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified Block 1 with deletion in E1 and insertion of GFP expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMK437 (mAID-mCherry-P2A-BSD)

Plasmid

#168232

Purpose

C-terminal tagging with mAID-mCherry

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-mCherry-P2A-BSD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-PSmOrange

Plasmid

#31886

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Expression

Bacterial

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YFP

Plasmid

#42225

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Use

Tags

TS:YFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pMK434 (mAID-mClover-P2A-Hygro)

Plasmid

#168229

Purpose

C-terminal tagging with mAID-mClover

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-mClover-P2A-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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BoNT/A-LC

Plasmid

#31602

Depositor

Axel Brunger

Article

Zuniga et al Structure. 2008 Oct 8. 16(10):1588-97.

Insert

BoNT/A LC

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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LRG2.1-TagBFP2

Plasmid

#124773

Purpose

Lentiviral plasmid to co-express a guide RNA and TagBFP2

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPT24-mtnluc

Plasmid

#184339

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Insert

nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:Xen5

Plasmid

#88902

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + LuxABCDE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
pMRX-No-HaloTag7-LC3

Plasmid

#184900

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP2834

Plasmid

#160673

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

Plasmid

#62348

Purpose

An empty optimized gRNA expression vector

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

none

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Mito-HyPerGRt

Plasmid

#173198

Purpose

AAV transfer plasmid for a HyPer7 and tdTomato fusion under hSyn promoter

Depositor

Huiwang Ai

Article

Li et al ACS Sens. 2022 Feb 28. doi: 10.1021/acss

Insert

Hyper7-tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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RTB300

Plasmid

#80410

Purpose

human cTNT minigene with genomic segment containing alternative exon 5

Depositor

Thomas Cooper

Article

Philips et al Science. 1998 May 1;280(5364):737-41.

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

RSV

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pEiLOV-N1

Plasmid

#26588

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA TrkB

Plasmid

#24088

Depositor

Moses Chao

Article

Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

Insert

TrkB (Ntrk2 Rat)

Use

Tags

Expression

Mammalian

Mutation

R133I

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-5u

Plasmid

#13554

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi S52A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

S52A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pPSmOrange-C1

Plasmid

#31899

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP SV2A GFP

Plasmid

#218544

Purpose

Mamallian Expression of SV2A GFP

Depositor

Edwin Chapman

Article

Dong et al Science. 2006 Apr 28;312(5773):592-6. do

Insert

SV2A

Use

Tags

Expression

Mammalian

Mutation

S42F

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

Plasmid

#71485

Purpose

Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRha-rsw-EYFP

Plasmid

#182928

Purpose

EYFP driven by inducible promoter Prha-rsw

Depositor

Himadri Pakrasi

Article

Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

Insert

EYFP

Use

Tags

Expression

Mutation

Promoter

Prha-rsw

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet

Plasmid

#130509

Purpose

expresses mScarlet

Depositor

Dorus Gadella

Article

Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

Insert

mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBGK

Plasmid

#65796

Purpose

Vector for Cas9 mRNA synthesis

Depositor

Masato Ohtsuka

Article

Harms et al Curr Protoc Hum Genet. 2014 Oct 1;83:15.

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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sspB-mSc-sspB

Plasmid

#114422

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-proD/Lux-nat

Plasmid

#120324

Purpose

Bacterial luminescence

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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