We narrowed to 8,811 results for: Ott
-
Plasmid#110638PurposeRetroviral expression of Flag-SFXN4 in mammalian cellsDepositorInsertSFXN4 (SFXN4 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70
Plasmid#78307PurposeCreating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-nlsLexA::GADflluw
UseTagsExpressionMutationPromoterAvailable sinceJuly 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
IZ401: pMVP/Lenti/Neo-DEST
Plasmid#121850PurposepMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassetteDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry2-sgC9cen
Plasmid#198326PurposeExpresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporterDepositorInsertChr9-CEN guide RNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV TBG FFluc miR122sponge
Plasmid#35657DepositorInsertmiR-122 sponge
UseAAVTagsExpressionMutationPromoterTBGAvailable sinceJuly 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
D44_Ld_180090
Plasmid#135858PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertalpha glucosidase II subunit, putative
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
KK402: pMVP/Zeo-DEST
Plasmid#121859PurposepMVP destination vector, empty expression vector backbone w/ Zeo selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJet-CMV-CD5HA2-bglpA
Plasmid#96962PurposeExpression plasmid for HA-tag embedded into human CD52 with short polyADepositorInsertCMV-CD5HA2-bglpA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAftsI
Plasmid#149590Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
KP201: pMVP (L5-L4) eGFP-P2A
Plasmid#121708PurposepMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.DepositorInserteGFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral CaMKKbeta K193A
Plasmid#33323DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFlagExpressionMutationK194APromoterhuman PGKAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-rev-g2-2A-Venus
Plasmid#71410PurposeThis plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.DepositorInsertsUseAAV and Cre/LoxTagsExpressionMammalianMutationPromotermouse hdc gene promoter, works as a pan neuronal …Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-rev_U6_MCS
Plasmid#109769PurposeMultisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequenceDepositorInsertU6-gRNA cassette
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
KP301: pMVP (L5-L4) mCherry-P2A
Plasmid#121710PurposepMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.DepositorInsertmCherry-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-SsOGT-H5-His
Plasmid#167277PurposeOverexpression of a sulfolobal equivalent of SNAP-tag in E. coliDepositorInsertSsOGT-H5
UseTagsHis x10 and Strep-tagExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST2-CeEP55
Plasmid#21501DepositorInsertCEP55-EABR (ESCRT and ALIX Binding Region) domain (CEP55 Human)
UseTagsGSTExpressionBacterialMutationQ230 (CAG changed to TAA), Q218 (CAA changed to T…PromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab8b
Plasmid#109658PurposeMuscle specific zebrafish expression of mKate2 fused to rab8bDepositorInsertrab8b (rab8b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab1ab
Plasmid#109606PurposeMuscle specific zebrafish expression of mKate2 fused to rab1abDepositorInsertrab1ab (rab1ab Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miRE_shBRD9_508-SV40-GFP
Plasmid#75138PurposeRetroviral expression plasmid encoding shBRD9_508 (targeting human BRD)DepositorInsertshBRD9_508
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
HE401: pMVP (L1-R5) shRNA
Plasmid#121698PurposepMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assemblyDepositorTypeEmpty backboneUseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-BTK-mKate2
Plasmid#109501PurposeMuscle specific zebrafish expression of mKate2 fused to the PH domain from Human Bruton tyrosine kinase (BTK). Binds PtdIns(3,4,5)P3.DepositorInsertactc1b-BTK-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Ptn in situ probe
Plasmid#45632DepositorInsertPtn in situ probe (Ptn Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 1241-1830 of NM_008973PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIRTetR-GAL80
Plasmid#185853PurposePlugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiaeDepositorInsertgal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
XW20
Plasmid#65836PurposePunc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-RhoAa
Plasmid#109525PurposeMuscle specific zebrafish expression of mKate2 fused to RhoAaDepositorInsertRhoAa (rhoaa Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-CaMKKbeta rat FL WT
Plasmid#33321DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsHAExpressionMammalianMutationS3V, G96R, and P108LPromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3E-FAPP1
Plasmid#109555PurposeMultisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4PDepositorInsertthe PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3).
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LF601: pMVP (L1-R5) TBG promoter
Plasmid#121695PurposepMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertTBG promoter (Liver specific)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD1b
Plasmid#109514PurposeMuscle specific zebrafish expression of mKate2 fused to EHD1bDepositorInsertEHD1b (ehd1b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAmreB
Plasmid#149594Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-LactC2
Plasmid#109499PurposeMuscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)DepositorInsertactc1b-mKate2-LactC2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab7a
Plasmid#109655PurposeMuscle specific zebrafish expression of mKate2 fused to rab7aDepositorInsertrab7a (rab7a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab7b
Plasmid#109656PurposeMuscle specific zebrafish expression of mKate2 fused to rab7bDepositorInsertrab7b (rab7b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LC402: pMVP (L3-L2) eGFP + polyA
Plasmid#121763PurposepMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.DepositorInserteGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab8a
Plasmid#109657PurposeMuscle specific zebrafish expression of mKate2 fused to rab8aDepositorInsertrab8a (rab8a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
KG802: pMVP (L3-L2) V5 epitope tag + polyA
Plasmid#121755PurposepMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertV5 epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-3xFlag-S242R-MEFV
Plasmid#167020PurposeGateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)DepositorInsertMEFV (MEFV Human)
UseGateway entry vectorTags3xFlagExpressionMutationserine 242 in arginine (S242R)PromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Foxp2 in situ probe
Plasmid#45620DepositorInsertFoxp2 in situ probe (Foxp2 Mouse)
UseIn situTagsExpressionMutationfragment contains bp#1709-2142 of AF339106PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
JN402: pMVP (R4-R3) Firefly Luciferase
Plasmid#121741PurposepMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertFirefly Luciferase
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYC-OspCas12c-noncoding
Plasmid#120878PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-OspCas12
UseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL V460P
Plasmid#61379PurposeExpresses Human full length RIPK3 with V460P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with V460Pmutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged V460 to PPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL V458P
Plasmid#61377PurposeExpresses Human full length RIPK3 with V458P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with V458Pmutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged V458 to PPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SauCas9KKH CCR5-nicking sgRNA
Plasmid#169863PurposeSauCas9KKH nicking sgRNA for CCR5DepositorInsertSauCas9KKH CCR5-nicking sgRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
KW701: pMVP (L5-L4) AID
Plasmid#121723PurposepMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest.DepositorInsertAID (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RELΔ424-490
Plasmid#27267DepositorInsertREL (REL Human)
UseTagsExpressionMammalianMutationDeletion of transactivation domain I (aa 425-490)PromoterAvailable sinceFeb. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
II901: pMVP (L5-L4) FKBP DD
Plasmid#121720PurposepMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.DepositorInsertFKBP Degron Domain (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ914
Plasmid#44489DepositorInsertMinimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
UseGateway expression cloneTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEnt R3L2 TetO(sh) mCh-Rs1-2
Plasmid#24417DepositorInsertRs1
UseEntry vectorTagsFLAG and mCherryExpressionMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
D92_Ld_360170
Plasmid#135905PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInserthypothetical protein, conserved
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only