We narrowed to 10,133 results for: transfer

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pPRIME-CMV-LNGFR-recipient

Plasmid

#11660

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-TetON-CD4

Plasmid

#217537

Purpose

AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptor

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

CD4 receptor (CD4 Human)

Use

AAV

Tags

HA epitope

Expression

Mammalian

Promoter

TetOperon (TetO)

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-R115A/R119A

Plasmid

#80696

Purpose

Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-R115A/R119A

Use

AAV

Expression

Mammalian

Mutation

Arg115 and Arg119 both mutated to Ala

Promoter

CBA

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2-CMV-GFP-BGHpA

Plasmid

#190239

Purpose

Expresses EGFP in mammalian cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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UPB-lung (UPB2)

Plasmid

#180788

Purpose

Unique Projection Barcode (UPB2)-Lung for Projection-seq

Depositor

Rui Chang

Article

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

humanized ChR2-H134R (partial)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209663

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

hSyn

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-CaMKII-TVA-P2A-N2cG

Plasmid

#172362

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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hSyn-GEM

Plasmid

#187648

Purpose

Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

PfV

Use

AAV

Tags

HaloTag and SNAP-tag

Expression

Mammalian

Promoter

Synapsin

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-Cas9

Plasmid

#166601

Purpose

Encodes Cre-dependent Cas9 under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

Cas9

Use

AAV

Promoter

tetracycline response element

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-H29A

Plasmid

#80602

Purpose

Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-H29A

Use

AAV

Expression

Mammalian

Mutation

His29 mutated to Ala

Promoter

CBA

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(Gs)

Plasmid

#230011

Purpose

Expresses anti-mCherry PAGER(Gs) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1Dxs

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP

Plasmid

#32481

Purpose

Cre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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UPB-colon (UPB4)

Plasmid

#180786

Purpose

Unique Projection Barcode (UPB4)-Colon for Projection-seq

Depositor

Rui Chang

Article

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

hM3Dq ( partial )

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-Cre

Plasmid

#203844

Purpose

Flp-dependent Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mRuby3-2A

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-CheRiff-T2A-mCherry

Plasmid

#170275

Purpose

CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

CheRiff-T2A-mCherry

Use

AAV

Promoter

11xUAS with TATA-box minimal promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-ChR2-EGFP

Plasmid

#114216

Purpose

Expresses channelrhodopsin-EGFP fusion in CamKIIa+ neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Channelrhodopsin 2

Use

AAV and Mouse Targeting

Tags

EGFP

Expression

Mammalian

Promoter

CamKIIa 1.3 kb

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-DeltaJunD

Plasmid

#68547

Purpose

AAV expression of DeltaJunD

Depositor

Eric Nestler

Article

Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

Insert

DeltaJunD-IRES-GFP

Use

AAV

Expression

Mammalian

Mutation

truncated splice variant

Promoter

CMV

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only
flex-ChrimsonR-EYFP-Kv

Plasmid

#135319

Purpose

Cre-dependent expression of soma-targeted ChrimsonR

Depositor

Hongkui Zeng

Article

Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

Insert

ChrimsonR

Use

AAV

Tags

EYFP

Promoter

synapsin

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)

Plasmid

#213402

Purpose

douple floxed, myristoylation (myr) site for membrane targeting and EGFP

Depositor

Katrin Willig

Article

Wegner et al Elife. 2022 Feb 23;11:e73603. doi: 10.75

Insert

EGFP

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-smURFP-P2A-GFP

Plasmid

#197151

Purpose

Expresses the protein of smURFP-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

smURFP-P2A-GFP

Use

AAV

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-fPV-GFP

Plasmid

#22914

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu parvalbumin promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pBK1289-AAV-dCjCas9

Plasmid

#223138

Purpose

AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)

Plasmid

#230024

Purpose

Expresses anti-TNFalpha PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204971

Purpose

Soma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

raACR

Use

AAV

Tags

mCerulean3

Promoter

CaMKII

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-UAS-anti-CD3/anti-CD19 BiTE

Plasmid

#230005

Purpose

Gal4-dependent expression of secreted BiTE

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

UAS-anti-CD3/anti-CD19 BiTE

Use

AAV

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-WPRE

Plasmid

#127860

Purpose

pAAV plasmid expressing mCherry under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

mCherry

Use

AAV

Promoter

hSyn

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_S34F_U2AF1

Plasmid

#176592

Purpose

Adenoviral construct to change S to F at 34th amino acid (S34F) in human U2AF1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

U2AF1 (U2AF1 Human)

Use

AAV

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-oG-WPRE-hGH

Plasmid

#74290

Purpose

pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Article

Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

Insert

oG (optimized Glycoprotein)

Use

AAV

Expression

Mammalian

Mutation

chimeric glycoprotein

Promoter

Ef1a

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-FLPo-T2A-GFP

Plasmid

#161766

Purpose

express FLPo and GFP under the control of human synapsin promoter

Depositor

Baoji Xu

Article

An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

Inserts

FLPo
T2AEGFP

Use

AAV

Expression

Mammalian

Promoter

hSynapsin1

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP680-P2A-GFP

Plasmid

#197164

Purpose

Expresses the protein of miRFP680-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680-P2A-GFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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