We narrowed to 19,679 results for: ACE

1. pEPSA5Cas9syn2

   Plasmid

   #206963

   Purpose

   S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

   Depositor

   Daijie Chen

   Article

   Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET51b(+)-SNAP-CLIP

   Plasmid

   #127180

   Purpose

   Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

   Insert

   SNAP-CLIP
   

   Use

   Tags

   HisTag and StrepTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFLAG-CMV4-ZIKV-NS3

   Plasmid

   #178844

   Purpose

   Mammalian expression of ZIKV ORF

   Depositor

   Judy Lieberman

   Article

   Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

   Insert

   ZIKV-NS3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pM1s3AsR_TS-C7

   Plasmid

   #137945

   Purpose

   E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pP2-P1(S')

   Plasmid

   #196334

   Purpose

   Plasmid expressing chimeric P2-P1(S') tail fiber

   Depositor

   Baojun Wang

   Article

   Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

   Insert

   chimeric tail fiber P2-P1(S') and chaperone protein U'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P2 late promoter

   Available since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. phGR-SB

   Plasmid

   #133409

   Purpose

   overexpression of a human glucocorticoid receptor in mammalian cells

   Depositor

   Andrew Cato

   Article

   Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

   Insert

   NR3C1 (NR3C1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mNeonGreen-IETD-NanoLuc

   Plasmid

   #98288

   Purpose

   caspase-8 BRET Sensor

   Depositor

   Maarten Merkx

   Article

   den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

   Insert

   mNeonGreen-LEHD-NanoLuc
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG

   Plasmid

   #139961

   Purpose

   Over-expression of heterologus squalene epoxidase from Nannochloropsis oceanica

   Depositor

   Raffaela Abbriano

   Article

   Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

   Insert

   ConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNICE-LAP-neurexin-1beta

   Plasmid

   #42575

   Depositor

   Alice Ting

   Article

   Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

   Insert

   LAP-neurexin-1beta
   

   Use

   Tags

   c-Myc, after signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET26b_Nb.AT110.i1

    Plasmid

    #135481

    Purpose

    E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110.i1
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYSD300

    Plasmid

    #212930

    Purpose

    Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    AGA1 (AGA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SPOON/ pcDNA3

    Plasmid

    #124840

    Purpose

    mammalian expression of SPOON, a spontaneously blinking green fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Arai et al ACS Chem Biol. 2018 Aug 17;13(8):1938-19

    Insert

    SPOON
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWXLd-RIEP-GpNLuc

    Plasmid

    #135935

    Purpose

    Expresses the GpNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. qTAG-N-Zeo-V5

    Plasmid

    #207708

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pBP-SJM901

    Plasmid

    #72966

    Purpose

    Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM901 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICH51288

    Plasmid

    #50269

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ607-CMV-GFP-2A-Cas9

    Plasmid

    #154830

    Purpose

    GFP-2A-Cas9 (CHO codon-optimized)

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    GFP-2A-Cas9
    HygR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized (Cricetulus griseus)

    Promoter

    CMV and SV40 promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJL-SaBE3

    Plasmid

    #85169

    Purpose

    Expresses SaBE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    SaBE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. G783A_pLenti-Tet-BattP(GA)-miRFP670-2A-iCasp9-2A-Hygro_rtTA3

    Plasmid

    #200635

    Purpose

    Negative selection hygromycin resistant Tet-inducible Matreyek Bxb1(GA) landing pad for dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    iCasp9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTRE3G

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRS-LacI-GFP

    Plasmid

    #220198

    Purpose

    mini-Tn7 GFP expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    LacI/pLac

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSpyC-GFP

    Plasmid

    #220191

    Purpose

    sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMRS-LacI-dCas9

    Plasmid

    #220194

    Purpose

    mini-Tn7 dCas9 expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    LacI/pLac

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTU424

    Plasmid

    #178983

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. qTAG-C-3xHA-Zeo

    Plasmid

    #207740

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. qTAG-N-Zeo-3xHA

    Plasmid

    #207707

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. ChlorON-3

    Plasmid

    #193479

    Purpose

    Turn-on fluorescent sensor for chloride

    Depositor

    Sheel Dodani

    Article

    Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

    Insert

    ChlorON-3
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTE105

    Plasmid

    #59400

    Purpose

    MethyloBrick expression vector, tuf promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptuf

    Available since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. apt-CBE-3'end

    Plasmid

    #219939

    Purpose

    Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    MCP_32-aa_evo-rA1_2xUGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. nCas9-SpRY_P2A-mCherry

    Plasmid

    #219935

    Purpose

    Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-SpRY_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 3TERM_35S

    Plasmid

    #136114

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_35S (CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pYR69 (X-4:4xUAS-SSA1p-365:yEGFP3-CYC1t)

    Plasmid

    #167355

    Purpose

    Integrative plasmid for promoter-reporter 4xUAS-SSA1p-365:yEGFP3-CYC1t

    Depositor

    Michael Jensen

    Article

    Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

    Insert

    4xUAS-SSA1p-365:yEGFP3-CYC1t
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGilesSHdI_psmyc_His6_triple red

    Plasmid

    #170047

    Purpose

    Mono-Responsive Reporter Gene Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR

    Plasmid

    #200636

    Purpose

    Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RIB VP64 R1

    Plasmid

    #161493

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRF0G-Tyr

    Plasmid

    #113644

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    MjYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pUPD2+pEstrInd

    Plasmid

    #170880

    Purpose

    Phytobrick - Estradiol Inducible Promoter

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    pEstradiol_Inducible
    

    Use

    Tags

    Expression

    Plant

    Mutation

    c.837C>T and c.1353G>A to remove BsaI and E…

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GR A458T

    Plasmid

    #133410

    Purpose

    overexpression of a mutant human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A458T

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFLAG-CMV4-ZIKV-NS4A

    Plasmid

    #178845

    Purpose

    Mammalian expression of ZIKV ORF

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

    Insert

    ZIKV-NS4A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEF1α-Cre-2A-miRFP670nano3

    Plasmid

    #207747

    Purpose

    Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-miRFP670nano3
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. mVenus-PA-PKI

    Plasmid

    #65456

    Purpose

    photoactivatable PKA inhibition

    Depositor

    Klaus Hahn

    Article

    Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

    Insert

    PKI (PKIA, Pkia Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only