We narrowed to 2,475 results for: T2A

1. pAK_DR30_EF1a_CasRx_Hygro

   Plasmid

   #134841

   Purpose

   Lentiviral all in one expression of CasRx and guideRNA

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR and Lentiviral

   Tags

   T2A Hygromycin

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAdx-CMV-iCre-P2A-copGFP

   Plasmid

   #73350

   Purpose

   Express iCre and GFP

   Depositor

   Kazuhiro Oka

   Insert

   CMV-copGFP-T2A-iCre
   

   Use

   Adenoviral

   Promoter

   CMV

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2-Synapse reporters

   Plasmid

   #196899

   Purpose

   zebrafish expression

   Depositor

   Reinhard Köster

   Insert

   Synapse reporters
   

   Use

   Zebrafish expression

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-DIO-HTB

   Plasmid

   #44187

   Depositor

   Edward Callaway

   Insert

   Histone tagged GFP, T2A-TVA-E2A-B19G
   

   Use

   AAV; Adeno-associated vector

   Tags

   GFP

   Promoter

   EF1a

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

5. DONOR plasmid

   Plasmid

   #236552

   Purpose

   Contains the insert sequence IRES-HaloTag-T2A-PuroR flanked by microhomologies for incorporation downstream of HSPA5 (BiP/GRP78).

   Depositor

   Pablo Rivera-Fuentes

   Insert

   HaloTag
   

   Expression

   Mammalian

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-dCas9-BFP-KRAB

   Plasmid

   #221502

   Purpose

   CRISPRi machinery fish codon optimized

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Mutation

   D10A and H839A point mutations

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA174_EF1a_EnAsCas12a_BFP

   Plasmid

   #196724

   Purpose

   Sortable and selectable EnAsCas12a.

   Depositor

   Bernhard Schmierer

   Insert

   enASCas12a-HA-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS - HA and SV40 NLS

   Mutation

   E174R, S542R, K548R

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Col2a1:TFPnls-mErt-Cre-Ert

   Plasmid

   #111152

   Purpose

   Chondrocyte specific inducible Cre

   Depositor

   Elly Tanaka

   Insert

   TFPnls-T2a-mErt-Cre-Ert
   

   Use

   Cre/Lox

   Mutation

   wt

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti.Cas9.BFP.Blast

   Plasmid

   #196714

   Purpose

   WT SpCas9 with BSD-TagBFP for selection and sorting

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-RfxCas13d-Blast-BFP

    Plasmid

    #196726

    Purpose

    Sortable and selectable RfxCas13d.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid

    #221503

    Purpose

    CRISPRi machinery fish codon optimized expressing plasmid

    Depositor

    Alessandro Ori

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    Fish expression

    Mutation

    D10A and H839A point mutations

    Promoter

    Zebrafish Ubiquitin promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti_P2A_Blast

    Plasmid

    #211365

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHACK-GAL4>GAL80

    Plasmid

    #104874

    Purpose

    HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.

    Depositor

    Christopher Potter

    Insert

    T2A-GAL80
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. StarShip01

    Plasmid

    #164505

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Availability

    Academic Institutions and Nonprofits only

    

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15. StarShip02

    Plasmid

    #164506

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Availability

    Academic Institutions and Nonprofits only

    

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16. StarShip03

    Plasmid

    #164507

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-tetO-NB

    Plasmid

    #197092

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-GFAP-TurboID

    Plasmid

    #227179

    Purpose

    Co-expression of FLAG-tagged-TurboID and T2A-mCherry under the GFAP short promoter by AAV vector

    Depositor

    Junmin Peng

    Article

    Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

    Insert

    TurboID
    

    Use

    AAV

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Promoter

    GFAP_short

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-CaMKII-TurboID

    Plasmid

    #227178

    Purpose

    Co-expression of FLAG-tagged TurboID and T2A-mCherry under the CaMKIIα short promoter by AAV vector

    Depositor

    Junmin Peng

    Article

    Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

    Insert

    TurboID
    

    Use

    AAV

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CaMKIIa_short

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-TetON-mEGFP-NGN2_WT

    Plasmid

    #215610

    Purpose

    For integration of NGN2 WT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    NGN2 (NEUROG2 Human)
    

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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