We narrowed to 8,983 results for: ef1;

1. Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75241

   Purpose

   CRISPR/Cas9 plasmid against human Ikaros

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Ikaros (IKZF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194283

   Purpose

   EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-EF1a-Archon2-KGC-EGFP-ER2

   Plasmid

   #108426

   Purpose

   Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon2-KGC-EGFP-ER2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a DIO-hsChRmine-oScarlet-WPRE

   Plasmid

   #183526

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   hsChRmine-oScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   H33R

   Promoter

   Ef1a

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L

   Plasmid

   #122991

   Purpose

   Expresses Flag-tagged hRPGRIP1L in mammalian cells

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Insert

   RPGRIP1L (RPGRIP1L Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a DIO-ChRmine-oScarlet-WPRE

   Plasmid

   #183524

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   ChRmine-oScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

   Plasmid

   #191397

   Purpose

   sgRNA targeting alpha-satellites on human chromosome 9

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   sgRNA targeting alpha satellites on human chromosome 9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-EF1a-CDK6-mutPAM-PGK-puro

   Plasmid

   #187638

   Purpose

   sg resistant CDK6 ORF

   Depositor

   William Kaelin

   Article

   Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

   Insert

   CDK6 (CDK6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   PAM site mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

   Plasmid

   #71218

   Purpose

   dCas9 fused to the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A(E752A)
   

   Use

   Lentiviral

   Tags

   SPdCas9-DNMT3A(E752A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177433

    Purpose

    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.

    Depositor

    David Andrews

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153538

    Purpose

    AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    EF1α1.1

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCF525-EF1a-Hygro-P2A-AcrVA4-lenti

    Plasmid

    #115663

    Purpose

    Expresses AcrVA4 in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA4
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO hChR2(E123A)-mCherry

    Plasmid

    #35508

    Purpose

    AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-GFP-SFPQY527A-IRES-mCherry

    Plasmid

    #166951

    Purpose

    Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    GFP and mCherry

    Expression

    Mutation

    SFPQ-Y527A

    Promoter

    eF1a

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-mir144 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164791

    Purpose

    Express miR-144 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144 (MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

    Plasmid

    #120268

    Purpose

    Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

    Depositor

    Edward Callaway

    Article

    Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

    Insert

    EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

    Plasmid

    #137165

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    3x-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    Ef1a

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

    Plasmid

    #164800

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA1
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

    Plasmid

    #164801

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Ef1a-Con/Foff 2.0-BFP

    Plasmid

    #137130

    Purpose

    Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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