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We narrowed to 7,494 results for: Tet-on

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Showing: 1681 - 1700 of 7494 results

  2. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Article
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  14. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Tags
    4FLAG
    Expression
    Bacterial
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193315-plasmid-map-sequence-id-382367

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  19. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid
    #202571
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202571-plasmid-map-sequence-id-429226

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  20. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100596-plasmid-map-sequence-id-207339

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Showing: 1681 - 1700 of 7494 results