We narrowed to 7,058 results for: tet on

1. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

   Plasmid

   #118593

   Purpose

   Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha/TRE3G

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

   Plasmid

   #227246

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α and pTRE3G

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17

   Plasmid

   #210020

   Purpose

   tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Oct4-p2a-Klf4-IRES-Sox2-17 (Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO-mini-CMV

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

   Plasmid

   #199349

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOEu

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

   Plasmid

   #227245

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α and pTRE3G

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

   Plasmid

   #199350

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOstar

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

   Plasmid

   #12153

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor (RHOA Human)
   

   Use

   Retroviral

   Tags

   6XHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

   Plasmid

   #166611

   Purpose

   Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   EGFP-2A-Tetanus Toxin
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag

   Plasmid

   #231594

   Purpose

   Express high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

   Depositor

   Marvin Tanenbaum

   Article

   Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

   Insert

   Kozak sequence-Tornado-10xSunTag-2xALFATag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

    Plasmid

    #78101

    Purpose

    Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-EED (EED Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

    Plasmid

    #199359

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc

    Plasmid

    #193349

    Purpose

    tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

    Plasmid

    #176630

    Purpose

    Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

    Plasmid

    #199348

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-TEV

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

    Plasmid

    #123657

    Purpose

    DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

    Depositor

    Stanley Qi

    Article

    Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

    Insert

    mCherry-P2A-DD-AcrIIA4
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    mCherry-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mU6

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

    Plasmid

    #101349

    Purpose

    2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta tat delta TAR delta env GFP in Nef Tet ope…

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

    Plasmid

    #78349

    Purpose

    Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR

    Plasmid

    #186740

    Purpose

    Dox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    RAB10, RABIF (RAB10, RABIF Human)
    

    Use

    Lentiviral

    Tags

    RAB10-T2A-RABIF-P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP

    Plasmid

    #199358

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2

    Plasmid

    #210019

    Purpose

    tetO-OKS (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + Sox2)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2 (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-mini-CMV

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge