164,909 results

1. pLacO-ISce1

   Plasmid

   #58505

   Purpose

   provides 256 Lac operator repeats adjacent to the ISce1 restriction site

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   Lac repeats adjacent to 6 copies of the ISce1 restriction site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   no promoter

   Available since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. IBMc396

   Plasmid

   #161954

   Purpose

   Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a-ValRS-His

   Plasmid

   #124122

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Valyl-tRNA ligase (valS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIN-TRE-EB3N-VVDfast-mCl3-iLID

   Plasmid

   #190169

   Purpose

   Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   EB3N-VVDfast-mClover3-iLID
   

   Use

   Lentiviral

   Tags

   -VVDfast-mClover3-iLID

   Expression

   Mutation

   1-189 aa from EB3

   Promoter

   TRE

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-hygro-STING V155M

   Plasmid

   #102599

   Purpose

   Express STING V155M for IFN-beta Luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

   Plasmid

   #164514

   Purpose

   Green glucose sensor with mRuby2 fusion, cytosolic

   Depositor

   Loren Looger

   Article

   Keller et al bioRxiv (2019) 571422; doi: https://doi.

   Insert

   (Cyto)iGlucoSnFR-mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Synthetic Construct

   Promoter

   GFAP

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit HC

   Plasmid

   #221349

   Purpose

   Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit HC; to be used with Anti-Biotin [IPI-M33] light chain (Plasmid 221350) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Biotin [IPI-M33] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa

   Plasmid

   #221350

   Purpose

   Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa; to be used with Anti-Biotin [IPI-M33] heavy chain (Plasmid 221349) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

   Plasmid

   #164611

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S383T

   Promoter

   CMV

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT076

    Plasmid

    #137880

    Purpose

    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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