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Addgene

We narrowed to 2,012 results for: ENDOG

Showing: 1741 - 1760 of 2012 results
  1. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Promoter
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. LLP617_αGCN4-mCherry

    Plasmid
    #211785
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection
    Depositor
    Insert
    SnoopCatcher-DNMT3A
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pUF1-dCas9-Sir2a

    Plasmid
    #122510
    Purpose
    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
    Depositor
    Insert
    dCas9-Sir2a
    Use
    CRISPR
    Tags
    3*FLAG
    Expression
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. Th-tdTomato

    Plasmid
    #159460
    Purpose
    Targeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassette
    Depositor
    Insert
    P2A-tdTomato
    Use
    Cre/Lox
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous TH
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pTriEX-Antenna-GDI Cdc42

    Plasmid
    #101681
    Purpose
    "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al
    Depositor
    Insert
    Cdc42
    Use
    Tags
    6xHis and FRET Antenna (mCerulean-cpVenus)
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV
    Available since
    March 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. LLP779_αGCN4-VP64-CO

    Plasmid
    #211772
    Purpose
    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection
    Depositor
    Insert
    aGCN4-VP64
    Use
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect VP64 e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. LLP790_αGCN4-EZH2-FL-CO

    Plasmid
    #211784
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection
    Depositor
    Insert
    SnoopCatcher-KRAB
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pONSY-mCherry

    Plasmid
    #111874
    Purpose
    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Insert
    mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1741 - 1760 of 2012 results