We narrowed to 1,917 results for: FRT

1. 5'-BirA-FLAG Kdm1a+exon minimal CMV pCDNA5

   Plasmid

   #212089

   Purpose

   LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells (BioID vector)

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Kdm1a+exon(12nt) (Kdm1a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5'-BirA-FLAG Chtop+exon minimal CMV pCDNA5

   Plasmid

   #212091

   Purpose

   LR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells (BioID vector)

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Chtop+exon(138nt) (Chtop Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_068

   Plasmid

   #164555

   Purpose

   Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_220

   Plasmid

   #164560

   Purpose

   Lentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Inserts

   First sgRNA cassette
   Second sgRNA cassette
   

   Use

   CRISPR and Lentiviral; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and Mouse U6

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRD443

   Plasmid

   #168469

   Purpose

   For the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mScarlet-I-H-NSdbd-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 10xHis-ataxin-3 *SIM-HA

   Plasmid

   #89983

   Purpose

   Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   10xHis and HA

   Expression

   Mammalian

   Mutation

   mutated aa 162IFVV to 162AFAA

   Promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOG44_FLP_recombinase (pEHA1338)

   Plasmid

   #209087

   Purpose

   FLP recombinase for FRT-mediated integration

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Insert

   FLP recombinase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3X FLAG-FUS-WT

   Plasmid

   #44985

   Depositor

   Robin Reed

   Article

   Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

   Insert

   FUS (Full length) (FUS Human)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

   Plasmid

   #105802

   Purpose

   Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-mRuby3; ORF2: TEV-mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

    Plasmid

    #154867

    Purpose

    Flp-dependent hM4D(Gi) DREADD-mCherry

    Depositor

    Ulrik Gether

    Article

    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTO_CHMP3_LAP

    Plasmid

    #101849

    Purpose

    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid

    #197264

    Purpose

    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

    Plasmid

    #197265

    Purpose

    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP

    Plasmid

    #137141

    Purpose

    Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Flp AND NOT Cre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-ChR2(ET/TC)-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E123T, T159C

    Promoter

    nEF

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTO_CHMP2B_LAP

    Plasmid

    #101848

    Purpose

    Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Flp-frt recombination, tet-inducible expression

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Mutation

    silent mutation in Thr 104; A to T

    Promoter

    CMV

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP

    Plasmid

    #137140

    Purpose

    Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChR2(ET/TC)-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E123T, T159C

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP

    Plasmid

    #137139

    Purpose

    Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-ChR2(ET/TC)-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E123T, T159C

    Promoter

    nEF

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

    Plasmid

    #122102

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-CAG-FLPX-rc [Chronos-tdTomato]

    Plasmid

    #105834

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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