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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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161,492 results

Showing: 241 - 270 of 161492 results

  7. pSBbi-Pur

    Plasmid
    #60523
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60523-plasmid-map-sequence-id-305758

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  8. pEG BacMam

    Plasmid
    #160451
    Purpose
    vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
    Depositor
    Eric Gouaux
    Article
    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160451-plasmid-map-sequence-id-319250

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  9. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Article
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Use
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86846-plasmid-map-sequence-id-172845

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  12. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  13. Multiplex Yeast Toolkit (MYT)

    Plasmid Kit
    #1000000229
    Purpose
    The Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids for genetically engineering the yeast, Saccharomyces cerevisiae.
    Depositor
    Tom Ellis
    Available since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only

  16. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

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  19. mApple-Alpha-V-Integrin-25

    Plasmid
    #54866
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Alpha-V Integrin (ITGAV Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54866-plasmid-map-sequence-id-330493

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  24. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

    Plasmid
    #44362
    Purpose
    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV; Adeno associated viral vector
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    April 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    44362-plasmid-map-sequence-id-292479

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  27. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV1)

    Viral Prep
    #105545-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    HI
    Tags
    EGFP
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  30. Tandem p40p35

    Plasmid
    #108665
    Purpose
    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker
    Depositor
    Nevil Singh
    Insert
    interleukin-12 (Il12a, Il12b Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    IL-12 dimers have a Gly-Ser linker
    Promoter
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    108665-plasmid-map-sequence-id-260875

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Showing: 241 - 270 of 161492 results