163,852 results

1. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV5)

   Viral Prep

   #162379-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Tags

   None

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

2. pZac2.1 gfaABC1D-tdTomato (AAV5)

   Viral Prep

   #44332-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-tdTomato (#44332). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-tdTomato plasmid DNA. GfaABC1D-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al J Gen Physiol. 2013 Apr 15.

   Promoter

   Tags

   tdTomato

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pLIX_403

   Plasmid

   #41395

   Purpose

   Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-hM4D(Gi)-mCherry (AAV2)

   Viral Prep

   #50475-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Human sgRNA library Brunello in lentiGuide-Puro

   Pooled Library

   #73178

   Purpose

   Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Has Service

   Lentiviral Prep

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

7. eeBxb1

   Plasmid

   #222339

   Purpose

   Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved and engineered Bxb1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV2/1

   Plasmid

   #112862

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPRv2-mCherry

   Plasmid

   #99154

   Purpose

   Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

   Depositor

   Agata Smogorzewska

   Inserts

   Cas9
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.TBG.PI.eGFP.WPRE.bGH (AAV8)

    Viral Prep

    #105535-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV.TBG.PI.eGFP.WPRE.bGH (#105535). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.eGFP.WPRE.bGH plasmid DNA. TBG-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    TBG

    Tags

    EGFP

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

11. CROPseq-Guide-Puro-mCherry2

    Plasmid

    #219679

    Purpose

    CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

    Depositor

    Christoph Bock

    Article

    Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV8)

    Viral Prep

    #44361-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

13. MoClo Tool Kit

    Plasmid Kit

    #1000000044

    Purpose

    Eukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminators

    Depositor

    Sylvestre Marillonnet

    Available since

    April 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV PHP.eB)

    Viral Prep

    #44362-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pPSU1

    Plasmid

    #89439

    Purpose

    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders

    Depositor

    Song Tan

    Article

    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

    Inserts

    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPSU2

    Plasmid

    #89566

    Purpose

    High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders

    Depositor

    Song Tan

    Article

    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

    Inserts

    750 bp EcoRV fragment
    3000 bp EcoRV fragment
    4000 bp EcoRV fragment
    50 bp PstI fragment
    100 bp PstI fragment
    200 bp PstI fragment
    300 bp PstI fragment
    400 bp PstI fragment
    500 bp PstI fragment
    600 bp PstI fragment
    1500 bp Psti fragment
    4100 bp PstI fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV1)

    Viral Prep

    #44361-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-hSyn-DIO-mCherry (AAV9)

    Viral Prep

    #50459-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pBS1C

    Plasmid

    #55168

    Purpose

    Empty vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-EGFP (AAV PHP.eB)

    Viral Prep

    #50465-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

21. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Use

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pGP-AAV-syn-jGCaMP8s-WPRE (AAV9)

    Viral Prep

    #162374-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Tags

    None

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

23. pEGFP-D50 lamin A

    Plasmid

    #17653

    Depositor

    Tom Misteli

    Article

    Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

    Insert

    D50 lamin A (LMNA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta 50 (50 aa internal deletion in globular tai…

    Promoter

    CMV

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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24. TRE3G-PE2-P2A-BFP

    Plasmid

    #231580

    Purpose

    Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

    Depositor

    Jesse Engreitz

    Article

    Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

    Insert

    PE2-P2A-BFP
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. ABE8e(TadA-8e V106W)

    Plasmid

    #138495

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. Laconic/pcDNA3.1(-)

    Plasmid

    #44238

    Purpose

    a genetically-encoded sensor for lactate concentration, transport and metabolic flux

    Depositor

    Luis Felipe Barros

    Article

    San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

    Insert

    Laconic (LACtate Optical Nano Indicator from CECs)
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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27. mCherry-SEpHluorin

    Plasmid

    #32001

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    mCherry
    

    Use

    Tags

    Superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDR111_Bgal no NLS

    Plasmid

    #188397

    Purpose

    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Phyper spank

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. TFORF0373

    Plasmid

    #141594

    Purpose

    Lentiviral vector for overexpressing the NFYA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFYA (NFYA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLJM1-EGFP-GRAM-H

    Plasmid

    #211703

    Purpose

    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (pLJM1-EGFP-GRAM1b G187L)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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