We narrowed to 8,918 results for: Pol

1. pGL3basic luciferase and renilla_polyA

   Plasmid

   #128046

   Purpose

   pGL3 basic luciferase reporter with co-expression of renilla luciferase

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-CMV-polysite

   Plasmid

   #122843

   Purpose

   Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7-CMVtrans-FFLuc-polyA

   Plasmid

   #101156

   Purpose

   Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

   Depositor

   Marcel Bruchez

   Article

   Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOpen-GBDpol (exo)

   Plasmid

   #165572

   Purpose

   Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pyEvolvR (enCas9-PolI5M)

   Plasmid

   #158073

   Purpose

   Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber

   Article

   Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

   Insert

   enCas9-PolI5M
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. hSyn-BiPOLES-mCerulean

   Plasmid

   #154944

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   BiPOLES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. hSyn-somBiPOLES-mCerulean

   Plasmid

   #154945

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6-AN-HA_WT POLK

   Plasmid

   #201584

   Purpose

   Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   Polymerase kappa (POLK Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6-AN-HA_WT POLH

   Plasmid

   #201671

   Purpose

   Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   Polymerase eta (POLH Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3e UbB polyA

    Plasmid

    #188702

    Purpose

    Gateway 3' element encoding the ubiquitin B polyadenylation sequence

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    UbB polyA (ubb Zebrafish)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. POLR2A-N CRISPR pX330

    Plasmid

    #124495

    Purpose

    A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

    Depositor

    Masato Kanemaki

    Article

    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

    Insert

    POLR2A targeting CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. HO-pGAL-poly-KanMX4-HO

    Plasmid

    #51664

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. CaMKII-somBiPOLES-mCerulean

    Plasmid

    #154948

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Has Service

    AAV Retrograde, AAV5, and AAV9

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVENUS-N1-POLR2G-GFP

    Plasmid

    #86872

    Purpose

    To express POLR2G (RPB7) tagged with VENUS at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    POLR2G (POLR2G Human)
    

    Use

    Tags

    VENUS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-DDK_WT POLI

    Plasmid

    #131228

    Purpose

    Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Coding sequence has been codon optimised for expr…

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-HIRA-polyA

    Plasmid

    #59781

    Purpose

    Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    HIRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. mDlx-BiPOLES-mCerulean

    Plasmid

    #154946

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal Dlx

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOT2-poly-cis

    Plasmid

    #141177

    Purpose

    To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter

    Depositor

    Guiliang Tang

    Type

    Empty backbone

    Use

    RNAi and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV6-AN-DDK_UBM1*2* POLI

    Plasmid

    #131232

    Purpose

    Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains inactivating mutations in both ubiquitin…

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOpen-K12polLF (exo-)

    Plasmid

    #165522

    Purpose

    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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