We narrowed to 15,787 results for: gRNA

1. STAGR_gRNAScaffold_mU6

   Plasmid

   #102844

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert gRNAScaffold_mU6
   

   Use

   Pcr template for stagr inserts

   Tags

   Expression

   Mutation

   Promoter

   mU6

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330_ATP1A1_G7_Dual_sgRNA

   Plasmid

   #173206

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

   Plasmid

   #197952

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pcoCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 and pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LCV2_LacZ_sgRNA_2

   Plasmid

   #155093

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT7tyrgRNA

   Plasmid

   #46761

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   tyr gRNA (tyr Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AY22_pgRNA.R5.1

   Plasmid

   #100294

   Purpose

   CCR5-targeting gRNA expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 RNA Pol III promoter human snRNA

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Rosa26_gRNA

   Plasmid

   #196138

   Purpose

   gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Rosa26 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. U6_sgRNA_CAG_hSt1Cas9_LMD9

   Plasmid

   #110626

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and hU6

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCBLsgRNA1

   Plasmid

   #199727

   Purpose

   Golden Gate entry vector; 1st gRNA under OsU3 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   OsU3-sgRNA1 scaffold
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCBLsgRNA2

    Plasmid

    #199728

    Purpose

    Golden Gate entry vector; 2nd gRNA under OsU3 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    OsU3-sgRNA2 scaffold
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. px458_2A_GFP_sgRNA_ZNF598

    Plasmid

    #127121

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA ZNF598 (ZNF598 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. T5-sgRNA

    Plasmid

    #113655

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter T5 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. gRNAU6

    Plasmid

    #135963

    Purpose

    Contains tracrRNA-hU6 sequence for double sgRNA cloning

    Depositor

    Yongming Wang

    Article

    Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSPneogRNAH

    Plasmid

    #63556

    Purpose

    Expresses gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    guide sequence
    

    Use

    Leishmania expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sgRNA1_ASCL1

    Plasmid

    #64129

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pColE1_sgRNA_M1S_AmpR

    Plasmid

    #119153

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pColE1_sgRNA_T2_AmpR

    Plasmid

    #119159

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pColE1_sgRNA_T3_AmpR

    Plasmid

    #119160

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pColE1_sgRNA_T1_AmpR

    Plasmid

    #119158

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pColE1_sgRNA_M1NS_AmpR

    Plasmid

    #119155

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge