165,637 results

1. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

   Plasmid

   #71237

   Purpose

   Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A GFP
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCG-TPMV Hd32

   Plasmid

   #163963

   Purpose

   Expresses TPMV H glycoprotein in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

   Insert

   TPMV H glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 2 to 33 at N terminus deleted

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SeAvs3

   Plasmid

   #188791

   Purpose

   Avs3 from Salmonella enterica NCTC13175

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   SeAvs3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC026

   Plasmid

   #227598

   Purpose

   Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTPH405c

   Plasmid

   #185726

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET41sIII_GST-ALC1

   Plasmid

   #173932

   Purpose

   Bacterial expression of ALC1

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1 (CHD1L Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCer-C3-Plk1-T210D

   Plasmid

   #68133

   Purpose

   Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells

   Depositor

   Catherine Lindon

   Insert

   Polo-like kinase 1 (PLK1 Human)
   

   Use

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   T210D constitutively active version

   Promoter

   CMV

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS mFgfr1 (CT#92)

   Plasmid

   #14005

   Depositor

   Cliff Tabin

   Insert

   Fgfr1 (Fgfr1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-STAT3mts

   Plasmid

   #195577

   Purpose

   Expresses STAT3 with mitochondrial targeting sequence

   Depositor

   Kevin Park

   Article

   Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

   Insert

   Signal transducer and activator of transcription 3 (Stat3 Mouse)
   

   Use

   AAV

   Tags

   Cox8 presequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer and promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRcCMV myc-Plk1 K82R (Nigg LA184)

    Plasmid

    #41157

    Depositor

    Erich Nigg

    Article

    Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

    Insert

    Plk1 (PLK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K82R

    Promoter

    CMV

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCAT112TAG-SepT

    Plasmid

    #34624

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    tRNA^Sep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. p354_DG

    Plasmid

    #234697

    Purpose

    Golden gate domesticated pET28b for scarless cloning with His10-TEV

    Depositor

    Matthew Wohlever

    Article

    Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTALYM16-Telo1

    Plasmid

    #47882

    Purpose

    TALE-mRuby2 against telomere sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCS4 3XFLAG-PPARgamma2

    Plasmid

    #78770

    Purpose

    To overexpress PPARgamma2 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPAR_2 (Ppargc1a Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pSPL3-hRPH3A_c.444Gwt

    Plasmid

    #190476

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138132

    Purpose

    Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEGFP-N1-LATS2

    Plasmid

    #166474

    Purpose

    Expresses fusion of mEGFP and LATS2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, LATS2 (LATS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1P-iCLHN

    Plasmid

    #66579

    Purpose

    AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Nanoluc-HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX-EF1a ANXA11-mEmerald D40G

    Plasmid

    #164211

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    D40G

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

    Plasmid

    #176278

    Purpose

    Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Kir2.1-P2A-EGFP (Kcnj2 Synthetic, Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. p21 promoter A

    Plasmid

    #16462

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAM5057

    Plasmid

    #120085

    Purpose

    Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

    Insert

    RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Hs.KRAS4b Q61H

    Plasmid

    #83140

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61H

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP_IRES_tdTomato

    Plasmid

    #184046

    Purpose

    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

    Depositor

    Angela Pannier

    Article

    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

    Inserts

    Enhanced Green Fluorescent Protein
    tdTomato
    Internal Ribosome Entry Site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBV65

    Plasmid

    #108363

    Purpose

    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

    Depositor

    Scott Moye-Rowley

    Article

    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

    Insert

    loxP-natMX6-CgMET3prom-cre-loxP
    

    Use

    Gene disruption marker for candida glabrata

    Tags

    Expression

    Mutation

    Promoter

    C. glabrata MET3

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.1(+)-OTI-TCRA

    Plasmid

    #131035

    Purpose

    Expresses OT-I TCRA

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD-MBP-His-SFP

    Plasmid

    #141141

    Purpose

    Expression of 4'-phosphopantetheinyl transferase gene

    Depositor

    New England Biolabs

    Insert

    4'-phosphopantetheinyl transferase
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Hs.KRAS4b G12V

    Plasmid

    #83132

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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