We narrowed to 7,871 results for: 199

1. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

   Plasmid

   #119946

   Purpose

   Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TetCMV

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CCR3-DuET

   Plasmid

   #213199

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR3 (CCR3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV.CAG.SF-iGluSnFR.A184V

   Plasmid

   #106199

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: A184V

   Promoter

   CAG

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF1891

   Plasmid

   #144199

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   WNK1 (WNK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKH3-TRIM28-C65A/C68A

   Plasmid

   #92199

   Purpose

   Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   Tif1b (TRIM28 Human, Synthetic)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Cysteines 65 and 68 to Alanines.

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SHC003BSD-EXOC7

   Plasmid

   #133298

   Purpose

   Expresses EXOC7 in mammalian cells

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Insert

   Exocyst complex component 7 (EXOC7 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV_T7-PE2-Nuclease

   Plasmid

   #171997

   Purpose

   Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CMV_T7-Cas9-RT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GC to CA point mutation in SpCas9 to restore nucl…

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF2248

   Plasmid

   #141990

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF12 (TCF12 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV302

   Plasmid

   #119943

   Purpose

   Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

   Depositor

   Gerald Schwank

   Article

   Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

   Insert

   N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV312.3

    Plasmid

    #119944

    Purpose

    Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid

    #140199

    Purpose

    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    Promoter

    PgpdA

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMVA2

    Plasmid

    #119951

    Purpose

    Expresses recombinant MVA pathway in Escherichia coli

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    mvaE
    mvaS
    mvaK1
    mvaK2
    mvaD
    idi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A110G

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-mCherry-p115

    Plasmid

    #181996

    Purpose

    Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mCherry-p115 (ARHGEF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Gene Trap - 24XPP7 Lentiviral Vector

    Plasmid

    #174199

    Purpose

    Lentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

    Depositor

    Daniel Larson

    Article

    Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

    Insert

    iRFP
    

    Use

    Lentiviral; Vector

    Tags

    24X PP7 hairpins, Blasticidin, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221_SLC26A11

    Plasmid

    #131996

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC26A11 (SLC26A11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pMYs-MFN2-IRES-mitoRFP

    Plasmid

    #121997

    Purpose

    Expression of MFN2 and mitoRFP in mammalial cells

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    MFN2-IRES-mitoRFP (MFN2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    silent Q386Q Q387Q V388V Y389Y C390C E391E E392E

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMEXneo-TrkC

    Plasmid

    #190202

    Purpose

    To express the Human TrkC receptor under the Moloney mouse sarcoma virus LTR.

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

    Insert

    TrkC (NTRK3 Human)
    

    Use

    Eucaryotic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney mouse sarcoma virus LTR

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-sgNotch1#1/Cre

    Plasmid

    #193223

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgNotch1#1 (Notch1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-sgNotch2#2/Cre

    Plasmid

    #193226

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgNotch2#2 (Notch2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-Puro-FH-AGO2-S831E

    Plasmid

    #91997

    Purpose

    Expresses FLAG-HA-AGO2 (S831E)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S831E

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge