Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 34,856 results for: CaS;

Showing: 1801 - 1820 of 34856 results

  2. 6xHis-MBP-huBsCas12a

    Plasmid
    #174671
    Purpose
    Expresses 6xHis-MBP tagged BsCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huBsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    174671-plasmid-map-sequence-id-364005

    Enlarge

  4. pAWPSG-Ptet-nCas9-BE

    Plasmid
    #195739
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet(Tetracycline inducible protein)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195739-plasmid-map-sequence-id-386424

    Enlarge

  6. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

    Enlarge

  9. pCasper4-g-osk-TRICK

    Plasmid
    #71244
    Purpose
    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.
    Depositor
    Anne Ephrussi
    Article
    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.
    Insert
    oskar (osk Fly)
    Use
    P element-mediated germline transformation
    Tags
    12xPP7 and 6xMS2
    Expression
    Insect
    Mutation
    Promoter
    osk
    Available since
    Dec. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    71244-plasmid-map-sequence-id-127267

    Enlarge

  10. 6xHis-MBP-huErCas12a

    Plasmid
    #174685
    Purpose
    Expresses 6xHis-MBP tagged ErCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huErCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis
    Expression
    Bacterial
    Mutation
    Q926R mutation is observed as indicated from the …
    Promoter
    T7
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    174685-plasmid-map-sequence-id-346198

    Enlarge

  15. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Article
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112065-plasmid-map-sequence-id-217190

    Enlarge

  17. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

    Enlarge

  18. pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

    Plasmid
    #176250
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176250-plasmid-map-sequence-id-346579

    Enlarge

Showing: 1801 - 1820 of 34856 results