We narrowed to 4,131 results for: p2a

1. pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169591

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO7-PCMVmin-2

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

   Plasmid

   #176279

   Purpose

   Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   GYG to AAA (aa144-146)

   Promoter

   human Synapsin I

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4

   Plasmid

   #193345

   Purpose

   tetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Oct4-p2a-Sox2-17-t2a-Klf4 (Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Neo-GFP/V5-Foxa1-(P2A)-Flag-Gata5

   Plasmid

   #105509

   Purpose

   MSCV-driven retroviral Foxa1 and Gata5 expression (P2A-linked, V5 and Flag-tagged each)

   Depositor

   Christopher Vakoc

   Article

   Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

   Inserts

   Foxa1 (Foxa1 Mouse)
   Gata5 (Gata5 Mouse)
   

   Use

   Retroviral

   Tags

   Flag and V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

   Plasmid

   #113687

   Purpose

   SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 (NEWENTRY )
   

   Use

   AAV and CRISPR

   Tags

   Flag, HAx2, KASH, and NLS

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

   Plasmid

   #113688

   Purpose

   SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 (NEWENTRY )
   

   Use

   AAV and CRISPR

   Tags

   Flag, HAx2, KASH, and NLS

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-SomArchon-mTagBFP2-p2A-CoChR-Kv2.1

   Plasmid

   #206006

   Purpose

   Expression of SomArchon-mTagBFP2 and CoChR under Syn promoter

   Depositor

   Xue Han

   Article

   Lowet et al Cell Rep. 2023 Aug 29;42(8):112906. doi:

   Insert

   SomArchon-mTagBFP2-P2A-CoChR-Kv2.1
   

   Use

   AAV

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP

   Plasmid

   #192609

   Purpose

   Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.

   Depositor

   Yingxiao Wang

   Article

   Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

   Insert

   LexA-CIB1-biLINuS-P2A-tagBFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   tagBFP

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

   Plasmid

   #173866

   Purpose

   Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101108

    Purpose

    Encodes dCas9(N) fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

    Plasmid

    #186745

    Purpose

    dCas9-10XGcn4-P2A-mCherry expression construct

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    Cas9-10XGcn4-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

    Plasmid

    #163644

    Purpose

    Makes a lentivirus that expresses mNeonGreen and Cas9.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    mNeonGreen-P2A-Cas9
    

    Use

    Lentiviral

    Tags

    mNeonGreen-P2A

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid

    #197505

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

    Promoter

    CMV and T7

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113689

    Purpose

    SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHIE067 CAG mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138747

    Purpose

    Integration Vector, assembled with pHIE041 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    CAG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBS1396 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]

    Plasmid

    #118804

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

    Plasmid

    #157951

    Purpose

    Lentiviral expression of HBG site 1 sgRNA

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-sfVenus-iGluSnFR

    Plasmid

    #187097

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-sfVenus-iGluSnFR
    

    Use

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-iRFP-P2A-Post-SPHERE-sfVenus-iGluSnFR

    Plasmid

    #187098

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-sfVenus-iGluSnFR
    

    Use

    Tags

    iRFP670-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138739

    Purpose

    Integration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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