Addgene

We narrowed to 26,789 results for: CAL

Showing: 1821 - 1840 of 26789 results

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pTET2-lucCP-3MS2

Plasmid

#198351

Purpose

Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Firefly Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

pTET2

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130997

Purpose

Double floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Expression

Mutation

Promoter

Ef1a

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

Plasmid

#130990

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHS1928

Plasmid

#205967

Purpose

JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH all proteins, with His-tagged Cas8

Use

Tags

14xHis on Cas8

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PPP1CA

Plasmid

#155843

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPP1CA (PPP1CA Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHS1862

Plasmid

#205975

Purpose

FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5 from JAGBLH010000220, Cas8-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1859

Plasmid

#205972

Purpose

FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Csy3/Cas7 from JAGBLH010000220, Cas8-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1860

Plasmid

#205973

Purpose

FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas6/Csy4 from JAGBLH010000220, Cas8-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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mEmerald(luminal)-canxTM

Plasmid

#186961

Purpose

Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CANX (CANX Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Only express calnexin (482-end) with ER signal pe…

Promoter

CMV Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130993

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Expression

Mutation

Promoter

hSyn

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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MIGR1-CD5

Plasmid

#159329

Purpose

Over-expression of mouse CD5

Depositor

Nevil Singh

Article

Matson et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

Insert

CD5 (Cd5 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2.0 Tag-RFP-T-UtrCH

Plasmid

#101279

Purpose

Life imaging pCS2.0 Tag-RFP-T_UtrCH

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

Utrophin (UTRN Human)

Use

Tags

TagRFP-T

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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RBM38

Plasmid

#155899

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM38 (RBM38 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHS1518

Plasmid

#205981

Purpose

FLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas6(Cse3/CasE) from MWEE01000013, Cas5-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHS1514

Plasmid

#205977

Purpose

FLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8/Cse1 from MWEE01000013, Cas5-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1515

Plasmid

#205978

Purpose

FLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas11/Cse2 from MWEE01000013, Cas5-HNH

Use

Tags

FLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1801

Plasmid

#205966

Purpose

JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH proteins and CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac (proteins), pJ23119 (crRNA)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1232

Plasmid

#205969

Purpose

MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH proteins and CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac (proteins), pJ23119 (crRNA)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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mEos2-Alpha-V-Integrin-25

Plasmid

#57345

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Catherine Galbraith, Michael Davidson

Article

Jaqaman et al Mol Biol Cell. 2016 May 15;27(10):1561-9

Insert

Alpha-V Integrin (ITGAV Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits